# TRANSPLANTATION ANTIGENS--EXPRESSION AND FUNCTION

> **NIH NIH R01** · UT SOUTHWESTERN MEDICAL CENTER · 1992 · $70,724

## Abstract

We plan to study the biology of class I genes encoded by the major
histocompatibility complex (MHC) of the inbred Balb/c mouse.
We have previously cloned and mapped 36 class I genes falling into
two categories-three classical transplantation genes which encode
the restriction elements for cytotoxic T cells and 33 class I-like
genes whose functions are unknown.  We plan three major
categories of experiments.  (1) To express a foreign
transplantation antigen (alloantigens) in nonimmunological tissues
(pancreas and liver) and in selected iummune-related cells (B cell,
T cell and macrophage-like cells).  These studies should provide
insights into mechanisms of immunological tolerance and H-2
restriction.  (2) To secrete and purify large quantities of soluble
transplantation antigens in order to crystallize these proteins and
determine their three-dimensional structures by X-ray analysis.
We will examine in this regard a series of Kb mutant molecules
and eventually a transplantation antigen interacting with a
peptide fragment.  These studies should provide insights into the
molecular interactions between H-2 molecules, T-cell receptors
and antigen.  (3) We have DNA probes specific for most of the
class I genes.  We plan to study the expression of the 36 class I
genes in immunological tissues and cells, in selected
nonimmunological tissues and in differentiating T and B cells and
in activated T cells.  These studies should provide useful lineage
and differentiation markers for immune-related cell lines and may
provide insights into the function of the class I-like molecules.

## Key facts

- **NIH application ID:** 3128964
- **Project number:** 7R01AI019624-11
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **SAM.gov UEI:** YZJ6DKPM4W63
- **Principal Investigator:** IWONA T STROYNOWSKI
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $70,724
- **Award type:** 7
- **Project period:** 1992-06-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128964

## Citation

> US National Institutes of Health, RePORTER application 3128964, TRANSPLANTATION ANTIGENS--EXPRESSION AND FUNCTION (7R01AI019624-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128964. Licensed CC0.

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