# H-2 LINKED COMPLEMENT PROTEINS BIOSYNTHESIS AND FUNCTION

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 1987 · $122,647

## Abstract

The specific aim of this project is to analyze the biosynthesis and
function-structure relationships of polymophic variants of H-2 linked
complement projects, C4, S1p, C2, and B.  We plan to continue one of our
long-term objectives, the genetics, regulation, function and structure of
mouse C4 and S1p.  This project will emphasize functional aspects of C4 and
S1p, especially as function relates to structure in polymorphic variants,
and the biosynthesis of C4 and S1p by short-term in vitro mouse peritoneal
macrophage and hepatocyte cultures.  The methodology primarily involves
biosynthetic labeling of C4 and S1p with radioactive sugar and amino acid
precursors, immunoprecipitation and subsequent analysis of the precipitated
complex by SDS-PAGE.  This modus operandi will also be applied to B since
antibodies are available and to C2 when the antibodies are produced.
Sensitive hemolytic assays are now available for C4, C2 and B and
polymorphic variants are known for each component.  In the previous four
years, much has been learned about the genetic regulation and
function-structure relationships of mouse C4. [An important observation,
that the secreted form of mouse and human C4 is about 5000 daltons larger
than the major form of C4 in plasma, will be analyzed in regards to the
protease responsible for the cleavage and function of the peptide
released.  We now plan to extend these studies to S1p, B and C2 while
continuing our work on C4.]  We believe our studies on C4 biosynthesis will
progress to the point that they will become an instructive model of the
evolution and control of a mammalian protein.  Through continued genetic,
structural and functional characterizations of these products, we hope to
gain better insights into the basis for their control by the major
histocompatibility complex.

## Key facts

- **NIH application ID:** 3128991
- **Project number:** 5R01AI019642-05
- **Recipient organization:** WASHINGTON UNIVERSITY
- **SAM.gov UEI:** L6NFUM28LQM5
- **Principal Investigator:** John Atkinson
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $122,647
- **Award type:** 5
- **Project period:** 1983-09-30 → 1988-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3128991

## Citation

> US National Institutes of Health, RePORTER application 3128991, H-2 LINKED COMPLEMENT PROTEINS BIOSYNTHESIS AND FUNCTION (5R01AI019642-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3128991. Licensed CC0.

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