# MOLECULAR STUDIES ON S TYPHIMURIUM ADHESIN

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1987 · $131,755

## Abstract

Adhesive properties which are essential for the invasion of animal cells
and for the virulence of orally administered S. typhimurium are encoded on
virulence plasmida of about 60 Mdal plasmid.  Our aim is to isolate and
characterize the adhesive substance and clone the adhesin gene for further
study.  Isolation will entail initial identification of unique envelopr
proteins of plasmid bearing strains by two-dimensional gel electophoresis.
The adhesin will be isolated by biochemical procedures coupled with the
production and use of monoclonal antibody in affinity chromatography.  The
adhesin gene will be cloned into suitable cloning vectors and transformed
into a S. typhimurium background for further study.  This will include
definitive studies on the role of adhesion employing insertionally
inactivated nonadhesive mutants, and the distribution of the salmonella
adhesin using cloned adhesin genes and specific adhesin antibody as
probes.  Cloning of the adhesive factor will enable us to define precisely
its function in interaction with animal cell (including macrophage)
receptors and the consequential induction of the animal cell's endocytic
mechanisms.  Of considerable interest is that in the mouse, resistance to
salmonellae, Mycobacteria and Leishmania is specified by a single locus
possibly involving macrophage activity.  The identification of functions in
resistance to salmonella will further studies on other intrcellular
pathogens in terms of how salmonellae enter and circumvent killing by
macrophages, and also provide needed insights of macrophage antibacterial
function.

## Key facts

- **NIH application ID:** 3129003
- **Project number:** 5R01AI019647-03
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** GARTH W JONES
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $131,755
- **Award type:** 5
- **Project period:** 1985-09-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129003

## Citation

> US National Institutes of Health, RePORTER application 3129003, MOLECULAR STUDIES ON S TYPHIMURIUM ADHESIN (5R01AI019647-03). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129003. Licensed CC0.

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