# WHITE BLOOD CELL OXIDASE - NORMAL AND ABNORMAL

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1992 · $214,172

## Abstract

Phagocytic cells undergo both metabolic and structural changes when
stimulated with either soluble or particulate stimuli.  These
changes, either spontaneously terminate after a short period of
time, or can be made to cease with removal of the stimuli.  The
overall goal of the proposed investigations for the next five years
are to gain a better understanding of the cellular and biochemical
aspects, of both reversible and irreversible inactivation of the
oxidase system responsible for killing bacteria, and for some of
the destructive ability of phagocytic cells.  We plan to determine
the kinetics of inactivation of both the whole cell superoxide
generating system and the particulate fraction - NADPH oxidase -
in both the reversible and irreversible manner, and in the presence
of various manipulations.  These studies will be performed in
granulocytes, moncytes and monocyte-derived macrophages.  We plan
to determine the cellular events that accompany both reversible and
irreversible inactivations concentrating on changes in
intracellular calcium, membrane potential an intracellular pH
together with phosphatidyl inositol metabolism and protein
phosphorylation.  Finally, we plan to determine the molecular and
biochemical events associated with inactivation of the oxidase
activated in subcellular particles, the PMA stimulated protein
kinase C dependent activity, and the arachidonic acid stimulated
soluble factor dependent activity.  In these studies we plan to
determine the effects of protein kinase C, calmodulin modifiers,
and GTP analogs on the inactivation process.  By examining cellular
and subcellular aspects of inactivation, we hope to gain a better
understanding of the control of this important process.

## Key facts

- **NIH application ID:** 3129015
- **Project number:** 5R01AI019656-10
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** HARVEY J COHEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $214,172
- **Award type:** 5
- **Project period:** 1983-01-01 → 1993-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129015

## Citation

> US National Institutes of Health, RePORTER application 3129015, WHITE BLOOD CELL OXIDASE - NORMAL AND ABNORMAL (5R01AI019656-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129015. Licensed CC0.

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