# BUNYAVIRUS REASSORTANTS IN VECTORS

> **NIH NIH R01** · COLORADO STATE UNIVERSITY · 1987 · $142,829

## Abstract

The goal of this research is to delineate the evolutionary potential and
gene-structure-biological function relationships of bunyaviruses in their
arthropod vectors at the organism, organ, and cell level.

The molecular events which occur during infection and transmission of
California serogroup viruses in their mosquito vectors will be determined.
These events will be examined at the level of replication and transcription
and translation of viral RNA species.  These experiments will entail the
production and use of a series of molecular reagents, including gene and
strand specific DNA probes and immune reagents specific for structural and
nonstructural virus proteins.  These reagents will permit us to follow the
course of viral RNA and protein synthesis within the vector and will permit
identification of aberrant viral RNA species which might arise during the
infection of the vector.  These aberrant RNA species may be potential
genomes of DI particles and may be involved in virus interference and
modulation.

The molecular basis of bunyavirus development in and transmission by
vectors will also be defined using parental and defined-clone RNA segment
reassortant viruses.  Determination of bunyavirus gene contributions to
vector-virus interactions should permit a better understanding of factors
operative in the maintenance of arbovirus cycles in nature.

The potential for RNA segment reassortment and generation of new
reassortant viruses will be determined in dually infected vectors.  In
addition, the potential for spontaneous bunyavirus mutation in persistently
(transovarially) infected vectors will be investigated.

Members of the Bunyaviridae are of considerable medical and veterinary
importance.  Determination of the evolutionary potential of the viruses by
RNA segment reassortment and by spontaneous mutation combined with
knowledge of the molecular events and genetic determinants of bunyavirus
infection and subsequent transmission by vectors might indicate which way
the epidemic potential of the viruses would evolve.

## Key facts

- **NIH application ID:** 3129068
- **Project number:** 5R01AI019688-06
- **Recipient organization:** COLORADO STATE UNIVERSITY
- **SAM.gov UEI:** LT9CXX8L19G1
- **Principal Investigator:** BARRY J BEATY
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $142,829
- **Award type:** 5
- **Project period:** 1982-07-01 → 1990-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129068

## Citation

> US National Institutes of Health, RePORTER application 3129068, BUNYAVIRUS REASSORTANTS IN VECTORS (5R01AI019688-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129068. Licensed CC0.

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