# GENE REGULATION IN CELLS INFECTED WITH HERPESVIRUSES

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1987 · $135,694

## Abstract

This is an application to dissect at the molecular level the virus
regulatory mechanisms that are responsible for controlling the replication
cycle of herpes simplex virus.  We propose to approach this problem by
constructing cell lines that contain and express individual members of the
immediate early gene family to deterine which of these genes has a role in
facilitating the transition from the synthesis of immediate early to early
transcription.  Cell lines containing these genes will be constructed by
transformation of primate and murine cells with selectable markers and a
cloned fragment of HSV DNA.  Cells containing and expressing the
cotransformed sequence will be identified on the basis of their ability to
support the replication of ts mutants and to promote transcription of
transfected virus DNA templates.  The availability of cell lines expressing
discrete virus functions will permit us to isolate mutants in these genes
and to begin to unravel their function.  A second aim of this application
is to use eukaryotic expression vectors to attempt to produce large amounts
of immediate early gene products.  This will facilitate purification of
these proteins so that we can begin to study their mechanism of action.  In
another study we propose to identify sequences that have been juxtaposed to
promoterless tk genes to ask why are these genes transcribed when cells
transformed with promoterless sequences are infected with HSV.  Finally, we
are constructing hybrid viruses that contain eukaryotic genes in the body
of the HSV chromosome.  These chimeric viruses are being used to introduce
foreign DNA sequences into eukaryotic cells and for studies on the
expression of eukaryotic sequences when they are cis to the virus
chromosome.

## Key facts

- **NIH application ID:** 3129096
- **Project number:** 5R01AI019724-05
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** SAUL J SILVERSTEIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $135,694
- **Award type:** 5
- **Project period:** 1983-07-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129096

## Citation

> US National Institutes of Health, RePORTER application 3129096, GENE REGULATION IN CELLS INFECTED WITH HERPESVIRUSES (5R01AI019724-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129096. Licensed CC0.

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