# T CELLS--FUNCTION AND ANTIGEN RECOGNITION

> **NIH NIH R01** · NATIONAL JEWISH HEALTH · 1992 · $211,527

## Abstract

The long term objective of this application is to understand the role of
gamma-delta T cells in the immune system.  Experiments proposed here are
designed to:

1) investigate the events leading to the selection of the gamma-delta T
cell repertoire: a substantial effort will be dedicated to the genetic
and structural characterization of the elements responsible for the
strain specific selective expansion of a model gamma-delta clonotype,
BID.   Various experimental approaches will involve formal genetics and
molecular biology.  BID T cell receptor transgenic mice and hematopoietic
chimeric mice will be used to complete these studies and to analyze the
cellular interaction leading to the selection of gamma-delta T cells.

2) further dissect the antigen specificities of gamma-delta T cells.  The
nature of the antigens and antigen presenting molecules for gamma-delta
T cells will be investigated. The relationship between mycobacterial
products and exogenous or self stress proteins will be assessed with the
help of T cell hybrids and monoclonal "genuine" gamma-delta T cell
populations, produced in transgenic mice in which a gamma-delta TcR which
recognizes mycobacterial antigens has been introduced.  The limits of the
self-non self discrimination process will be investigated in the
particular case of gamma-delta T cells.

3) analyze the function of gamma-delta T cells.  Effector functions such
as cytotoxicity and lymphokine production will be studied  using
polyclonal and monoclonal freshly isolated gamma-delta T cells following
TcR stimulation.  The possibility of gamma-delta T cells collaborating
with pulmonary epithelial cells in inducing IgA production in B cells
will be investigated.

Selective accumulation of gamma-delta T cells has been reported for human
pathological conditions ranging from coeliac disease, sarcoidosis of the
lung,  to lepromatous granulomas and rheumatoid arthritis.  The part
played by gamma-delta T cells in these lesions has not yet been
determined.  To do this, basic knowledge on the biology of gamma-delta
T cells has to be expanded.   Experiments proposed here are part of this
process.l

## Key facts

- **NIH application ID:** 3129185
- **Project number:** 5R01AI019775-11
- **Recipient organization:** NATIONAL JEWISH HEALTH
- **SAM.gov UEI:** VLQKFEEJ3NE9
- **Principal Investigator:** ANDREI A AUGUSTIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $211,527
- **Award type:** 5
- **Project period:** 1982-08-01 → 1994-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129185

## Citation

> US National Institutes of Health, RePORTER application 3129185, T CELLS--FUNCTION AND ANTIGEN RECOGNITION (5R01AI019775-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129185. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*