# THE ATP-COUPLED ARSENICAL PUMP OF ESCHERICHIA COLI

> **NIH NIH R01** · WAYNE STATE UNIVERSITY · 1990 · $150,535

## Abstract

The long-term objectives of this research is determination of the
molecular mechanisms of plasmid-encoded ATP-driven anion
pumps and the role of these transport systems in bacterial
resistance to antibiotics and toxic compounds.  The clinical
resistance plasmid R773 carries the arsenical resistance (ars)
operon, which codes for an ATP-linked arsenical extrusion pump
for arsenate (AsO43-) and arsenite (AsO2-).  Analysis will be
extended to determine the biochemical properties of the systems,
with the following specific aims.

1.  GENETICS AND MOLECULAR BIOLOGY:  As a
preliminary to biochemical studies of the transport systems, the
physical and genetic properties of the plasmid DNA will be
characterized.
2.  IDENTIFICATION AND PURIFICATION OF GENE
PRODUCTS OF THE ARS OPERON:  The ArsA and C have been
purified.  The ArsB protein, predicted from the nucleic acid
sequence to be a membrane protein, will be identified by
expression in the appropriate vectors, isolated, and purified.
3.  STRUCTURE-FUNCTION RELATIONSHIPS:  The
position of amino acyl residues of potential functional importance
in the Ars proteins will be predicted.  Indentifiable residues will
be changed by site-directed mutagenesis in order to map
functional domains within the subunits.
4.  DETERMINATION OF IN VITRO ACTIVITY:  The
catalytic activities of the arsenical pumps will be examined in
vitro.  ATP binding studies will be performed with the ArsA
protein, which, from its nucleotide sequence, is predicted to have
two nucleotide binding sites.

## Key facts

- **NIH application ID:** 3129213
- **Project number:** 5R01AI019793-07
- **Recipient organization:** WAYNE STATE UNIVERSITY
- **SAM.gov UEI:** M6K6NTJ2MNE5
- **Principal Investigator:** BARRY P. ROSEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $150,535
- **Award type:** 5
- **Project period:** 1987-08-01 → 1992-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129213

## Citation

> US National Institutes of Health, RePORTER application 3129213, THE ATP-COUPLED ARSENICAL PUMP OF ESCHERICHIA COLI (5R01AI019793-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129213. Licensed CC0.

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