# MAJOR HISTOCOMPATIBILITY ANTIGENS & MEMBRANE RECEPTOR

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1988 · $113,775

## Abstract

Our goal is to show the extent to which MHC antigens influence ligand
binding by peptide hormone receptors and in turn are modified as a
consequence of ligand binding to specific receptors.  We have shown as
association between HLA (genotype and phenotype) and the affinity of
insulin receptors on B lymphoblasts.  We will now further analyze the
genetic associations between HLA and insulin receptor affinity and extend
the analysis to the physical relationships between the antigens and
receptor  molecules.  The genetic analysis will proceed through examination
of mutant lymphoblasts, deleted for expression of some HLA antigens, which
have been transfected with genes for the missing antigens.  We will also
attempt to rescue expression of HLA antigens in Daudi cells, to examine the
effects of such rescue on insulin binding by Daudi.  The physical analysis
will take two approaches:  1.  an attempt, based on precedents in the
literature, to co-precipitate insulin receptors, detected by their
endogenous tyrosine kinase activity, with class I MHC antigens, using
specific anti-receptor and anti-class I monoclonal antibodies and 2.
determination of the proximity of class I MHC antigens and insulin
receptors in intact cells, using resonance energy transfer between
endogenously (Beta-2-m) labeled class I antigens, and insulin receptors
which we have shown can be specifically labeled with biotinyl insulin and
avidin phycobilliproteins.  Energy transfer will be measured in the flow
cytometer in terms of donor quenching sensitized emission of acceptor.

We plan to extend our work on the metabolic effects of epidermal growth
factor binding on the display and phosphorylation state of wild-type and
mutant class I MHC antigens, using flow cytometry to quantitate the display
and standard immunochemical and biochemical approaches to determine the
phosphorylation state of the antigens associated with changes in the
display.

## Key facts

- **NIH application ID:** 3129245
- **Project number:** 5R01AI019814-05
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** MICHAEL A EDIDIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $113,775
- **Award type:** 5
- **Project period:** 1983-07-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129245

## Citation

> US National Institutes of Health, RePORTER application 3129245, MAJOR HISTOCOMPATIBILITY ANTIGENS & MEMBRANE RECEPTOR (5R01AI019814-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129245. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*