# IMMUNOCHEMICAL STUDIES OF CHEMOATTRACTANT SUBSTANCES

> **NIH NIH R01** · JOHNS HOPKINS UNIVERSITY · 1988 · $145,402

## Abstract

Generation of chemoattractant substances is believed to play an important
role in the development and outcome of infectious and allergic processes.
Recent studies have attracted attention to chemoattractant activities of
substances such as LPC (lysophosphatidylcholine), HETEs (5-hydroxy or
12-hydroxy eicosatetraeonic acid) and leukotrienes which are derived from
membrane phospholipids.  Accordingly, we have initiated an immunological
and biochemical investigation with the purpose of:  (1) Conducting further
studies of lysophosphatidylcholine, a newly recognized chemoattractant for
lymphocytes, and (2) investigating a new aspect of complement-induced
inflammation by assessing the role of complement proteins C5b-9 in the
generation of LPC, HETEs, leukotrienes and, possibly, other
chemoattractants from membrane phospholipids.

With respect to LPC we want to determine what cell types respond to its
attractant activity, what role it plays in vivo as a mediator of
inflammation, what structural analogs of LPC exhibit chemoattractant
activity, whether LPC is converted to other chemoattractants by responding
cells, and whether LPC can induce formation of new chemoattractants
including some arachidonate metabolites with known chemoattractant
activity.  With respect to complement, we want to determine what cell types
produce chemoattractant factors when attacked by C5b-9 and to assess
formation of LPC, HETEs, leukotrienes and, possibly, other
chemoattractants.  We expect that these efforts will expand knowledge of
complement-induced inflammation beyond the well-known activities of
complement fragments C3a, C4a, C5a, and C3b.  We believe that activation of
complement at tissue sites of infection, allergy and malignancy, is likely
to lead to membrane damage which could activate membrane-associated
phospholipase and other enzymes that are instrumental in producing
chemoattractant phospholipid derivatives, including LPC.

## Key facts

- **NIH application ID:** 3129262
- **Project number:** 5R01AI019826-05
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **SAM.gov UEI:** FTMTDMBR29C7
- **Principal Investigator:** HYUN S SHIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $145,402
- **Award type:** 5
- **Project period:** 1983-12-01 → 1989-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129262

## Citation

> US National Institutes of Health, RePORTER application 3129262, IMMUNOCHEMICAL STUDIES OF CHEMOATTRACTANT SUBSTANCES (5R01AI019826-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129262. Licensed CC0.

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