# MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS

> **NIH NIH R01** · DUKE UNIVERSITY · 1987 · $132,677

## Abstract

The structure and function of the replication initiation protein of the
drug resistance factor R6K will be studied using biochemical and genetic
techniques with the long-term objectives of understanding the initiation
step of DNA replication.  The initiation of replication is the most
important step and perhaps the only step which regulates the orderly
replication of genes and, therefore, it has important implications for
understanding the control of gene duplication in normal and abnormal or
cancerous cells.  We have partially purified the replication initiation
protein of R6K by tagging it with Beta galactosidase which not only
provides a convenient marker but also stabilizes the initiator protein.
The tagged initiator has identical DNA-binding and replication initiation
activities as the nontagged, original initiator protein.  Using the
techniques of chemical protection, site specific mutagenesis and missense
suppression we aim to study the DNA protein interaction, between the
initiator and its binding site and between the initiator and other proteins
of the replisome.

## Key facts

- **NIH application ID:** 3129324
- **Project number:** 5R01AI019881-05
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** DEEPAK BASTIA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $132,677
- **Award type:** 5
- **Project period:** 1983-07-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129324

## Citation

> US National Institutes of Health, RePORTER application 3129324, MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS (5R01AI019881-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129324. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*