# MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS

> **NIH NIH R01** · DUKE UNIVERSITY · 1989 · $233,573

## Abstract

Our long term goals are to understand the mechanism of control
of initiation  of DNA replication in the plasmids R6K and RK2 and
to further investigate the alternative pathways of replication
initiation in RK2 recently discovered by us.  Our specific goals
are as follows:  (a) to investigate DNA looping in vivo and in vitro
in the activation of beta and alpha origins of R6K by initiator
protein bound to the gamma-enhancer; (b) to further investigate
the interaction of silencer RNA with activator RNA and ori DNA
and the role of initiator protein in these interactions; (c) to use
and antisence strategy to identify and map the activator (primer)
RNAs of alpha, beta and gamma origins.  Using various labeling
techniques, maps the RNA-DNA junctions in these putative
primer RNAs; (d) to use purified host proteins and in vitro
replication system to reconstitute R6K replication initiation in
vitro; (e) to purify the trfA, K or B and 13 Kd proteins of RK2 to
homogeneity and to study their interactions with the ori; (f) to
elucidate biochemcial properties of these proteins by using in
vitro replication system.  The analysis of the dnaA dependent and
independent pathways of initiation in RK2, recently discovered by
us, should illuminate the various strategies used by the broad host
range plasmid to initiate and control its replication in gram
negative hosts.

## Key facts

- **NIH application ID:** 3129325
- **Project number:** 5R01AI019881-07
- **Recipient organization:** DUKE UNIVERSITY
- **SAM.gov UEI:** TP7EK8DZV6N5
- **Principal Investigator:** DEEPAK BASTIA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $233,573
- **Award type:** 5
- **Project period:** 1983-07-01 → 1993-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129325

## Citation

> US National Institutes of Health, RePORTER application 3129325, MECHANISM OF REPLICATION OF DRUG RESISTANCE FACTORS (5R01AI019881-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129325. Licensed CC0.

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