# DEVELOPMENTAL DEFECTS AFFECTING HUMORAL IMMUNITY

> **NIH NIH R01** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 1990 · $243,830

## Abstract

Substantial progress has been recently made in understanding how
massive numbers of B lymphocytes are made daily within
mammalian bone marrow.  A number of differentiation events
have been identified which occur during the last several days of
this process.  Distinctive surface markers can be used to isolate
and characterize cells during this period and we already know of
several soluble mediators which influence their replication and
maturation.  Additional information of this kind should be
forthcoming in the near future and we plan to employ several new
methods and approaches to phenotyping such late B lineage
precursors.  However, the most important and least understood
issues relate to earlier events and relevant studies are planned
using genetically defective mice, long term culture methodology
and a variety of lympho-hemopoietic growth and differentiation
factors.  The progeny of multipotential stem cells simultaneously
progress through eight separate lineages while closely packed
within the hemopoietic cords of bone marrow.  It is important to
learn how these are coordinately regulated and how defects in one
lineage influence events in others.  A comprehensive study will be
done of various progenitor cells in a number of experimental
animal models and hypotheses will be tested about cells which
might have critical regulatory roles.  It is now practical to
investigate functional relationships between adherent stromal
cells and B lineage lymphocytes which depend on them for growth
in long term culture.  Soluble mediators as well as short range
communication may be involved.  In addition, adhesion molecules
must be important for appropriate sorting and migration of
differentiating cells within bone marrow.  It should be possible to
characterize ones which mediate recognition between lymphocyte
progenitors and microenvironmental cells in culture and
determine if this process is affected by genetically determined
defects.

## Key facts

- **NIH application ID:** 3129343
- **Project number:** 5R01AI019884-09
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **SAM.gov UEI:** NGCNCJ1X6XA4
- **Principal Investigator:** Paul Wayne Kincade
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $243,830
- **Award type:** 5
- **Project period:** 1982-08-01 → 1992-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129343

## Citation

> US National Institutes of Health, RePORTER application 3129343, DEVELOPMENTAL DEFECTS AFFECTING HUMORAL IMMUNITY (5R01AI019884-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129343. Licensed CC0.

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