# B CELL AND REPERTOIRE DEVELOPMENT IN VITRO

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · 1990 · $157,834

## Abstract

The goal of this project is to determine the mechanism of B
lymphocyte differentiation leading to a diverse immune repertoire
and how this process is regulated.  This will be accomplished under
defined conditions by the use of in vitro lymphocyte culture
systems including a fetal organ culture and long term lymphocyte
cultures.  B cell development and the associated diversity will be
assessed by the following criteria:  1) selection and expression
of VH and VL gene families at the single cell level by in situ
hybridization with radiolabelled VH and VL gene probes, 2) mitogen
responsiveness, 3) frequency of antigen specific B cells and
associated idiotypes using the splenic fragment assay, and 4)
acquisition of B lineage surface markers.  Because it is now
possible to assess V region expression in the unstimulated B cell
or pre B cell, important background information will be obtained.
The available VH and VL gene family repertoire, in the absence of
stimulation, will be precisely determined at various ages in
different tissues, and in different strains.  Evidence is presented
for distinct B cell progenitors in the adult vs fetus.  Experiments
outlined will determine the role distinct progenitors plays in B
cell formation and the generation of diversity.  In addition, the
effect of microenvironment on B cell differentiation and repertoire
development will be defined using cloned stromal cell lines
producing distinct B lineage growth and differentiation factors.
The effect of adding exogenous factors will also be determined.
Finally, parameters that affect the selection of the B cell
repertoire will be assessed.  These experiments should provide new
insights into B cell differentiation and the generation of
diversity, insights which should contribute to an understanding of
both cancer and autoimmunity.

## Key facts

- **NIH application ID:** 3129361
- **Project number:** 5R01AI019896-08
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
- **SAM.gov UEI:** C3KXNLTAAY98
- **Principal Investigator:** JUDY M TEALE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $157,834
- **Award type:** 5
- **Project period:** 1982-09-01 → 1993-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129361

## Citation

> US National Institutes of Health, RePORTER application 3129361, B CELL AND REPERTOIRE DEVELOPMENT IN VITRO (5R01AI019896-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129361. Licensed CC0.

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