# BACTERIAL IDENTIFICATION BY MULTIPARAMETER FLUORESCENCE

> **NIH NIH R01** · EMORY UNIVERSITY · 1987 · $65,915

## Abstract

The proposed research will use multiparameter fluorescence techniques to
achieve rapid, specific detection and identification of bacteria from
laboratory or clinical isolates.  Several components of the proposed
research will involve the use of a video fluorometer (VF), a highly
efficient instrument which simultaneously records both excitation and
emission spectra with a multichannel detector.  The VF can acquire up to
200 emission spectra excited at up to 200 different wavelengths in less
than one second.  Several fluorescent species can be detected
simultaneously taking advantage of the inherrently multicomponent nature of
fluorescence.  In this proposed study, the VF will be used to explore
identification procedures with fluorescent tagged antibodies specific for a
given bacteria and with identification procedures involving a series of
bacterial specific enzymes and fluorescent tagged substrates.  In addition,
the VF will be used to continue studies involving multiple dye
fingerprinting procedures where the adsorption characteristics of a
bacterium for a mixture of dyes is measured and used to categorize the
bacterium.  The development of data processing techniques will continue.
For example, two-dimensional Fourier Transform based pattern recognition
techniques will be used in conjunction with Mixed-Dye-Fluorometry.  Other
studies will explore the use of fluorescence detected circular dichroism
(FDCD) and fluorescence lifetime to provide expanded information about
fluorescent components of many bacterial types.

## Key facts

- **NIH application ID:** 3129379
- **Project number:** 5R01AI019916-05
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** ISIAH M WARNER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $65,915
- **Award type:** 5
- **Project period:** 1982-09-01 → 1988-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129379

## Citation

> US National Institutes of Health, RePORTER application 3129379, BACTERIAL IDENTIFICATION BY MULTIPARAMETER FLUORESCENCE (5R01AI019916-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129379. Licensed CC0.

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