# MOLECULAR AND GENETIC ANALYSIS OF PARVOVIRUS REPLICATION

> **NIH NIH R01** · YALE UNIVERSITY · 1991 · $236,788

## Abstract

The major objective of this proposal is to determine the
mechanism(s) of autonomous parvovirus transcription, at the
molecular and enzymatic level, using the fibrotropic (p) and
lymphotropic (i) variants of Minute Virus of Mice (MVM) and the
human B19 virus as model systems.  These viruses show a
remarkable target cell specificity for their replication, both in
vivo and in vitro.  In the case of MVM(p) and MVM(i), which are
reciprocially restricted in their ability to replicate in fibroblasts
and lymphocytes, recent studies have shown that the replication
block in restrictive host cells occurs at the level of transcriptional
initiation and demonstrated a requirement for a differentiation-
specific intra-nuclear "factor" for the establishment of active
viral transcription templates.  To further define the transcription
processes of these viruses a combination of genetic, biochemical
and immunological approaches will be taken to identify both the
genomic sequences and the viral or cellular proteins involved.
Particular attention will be paid to the characterization of the
various molecular forms of the MVM non-structural proteins NS-1
and NS-2, since one or more of these polypeptides, derived by
transcription off the P-4 promoter, functions as a transactivator
of the P-38 promoter.  Conventional and affinity chromatographic
techniques will be applied to the purification of those cellular
factors from murine and human cell so identified, and antibody or
oligonucleotide probes will be used to clone the genes for these
factors.  Mutational analysis of viral and cellular genes will be
done so as to identify the functional domains of each gene
product.  Such studies should hopefully provide further insights
into the regulation of viral gene expression in infected eukaryotic
cells.

## Key facts

- **NIH application ID:** 3129441
- **Project number:** 5R01AI019973-09
- **Recipient organization:** YALE UNIVERSITY
- **SAM.gov UEI:** FL6GV84CKN57
- **Principal Investigator:** DAVID C WARD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $236,788
- **Award type:** 5
- **Project period:** 1983-04-01 → 1993-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129441

## Citation

> US National Institutes of Health, RePORTER application 3129441, MOLECULAR AND GENETIC ANALYSIS OF PARVOVIRUS REPLICATION (5R01AI019973-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129441. Licensed CC0.

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