# STUDIES ON POLIOVIRUS USING INFECTIOUS VIRAL CDNA CLONES

> **NIH NIH R01** · COLUMBIA UNIV NEW YORK MORNINGSIDE · 1987 · $155,426

## Abstract

This proposal describes experiments that are concerned with the study of
poliovirus replication and pathogenesis.  An infectious viral cDNA clone
will be mutagenized to create a collection of altered plasmids which will
be used for most of the proposed studies.  The functions of the central
(P2) region of the genome and the 5'- and 3' -untranslated sequences will
be addressed by studying viable virus mutants containing alterations in
these regions.  Mutated, noninfectious cDNAs which contain lesions in these
areas of the genome will be studied by synthesizing positive strand RNA and
transfecting this RNA into cultured cells.  The role of terminal nucleotide
sequences of the viral RNA in translation and replication will be studied
using in vitro systems employing as templates mutated, subgenomic RNA
fragments.  Questions on the biology of poliovirus defective-interfering
particles will be addressed.  Positive strand RNAs will be synthesized
which contain deletions in the capsid region, and these RNAs will be
transfected into cells to determine whether DI RNAs can replicate, persist
in and be rescued from mammalian cells.  A mouse model for poliomyelitis
will be studied to identify viral functions which participate in the
production of paralytic disease.  Mouse-avirulent viral mutants will be
isolated and used to study the molecular basis of neurovirulence.  The
mouse model will also be used to identify viral capsid protein sequences
required for entry of poliovirus into cells of the mouse central nervous
system.  To better understand how the viral capsid interacts with a cell
during initiation of an infectious cycle, the cellular receptor for
poliovirus will be studied.  DNA transfection techniques will be used to
isolate molecular clones of the cellular gene encoding the poliovirus
receptor.  These studies are part of our long-term research goal, to
provide a complete description of the replication of a human pathogen.

## Key facts

- **NIH application ID:** 3129498
- **Project number:** 5R01AI020017-05
- **Recipient organization:** COLUMBIA UNIV NEW YORK MORNINGSIDE
- **SAM.gov UEI:** F4N1QNPB95M4
- **Principal Investigator:** VINCENT R RACANIELLO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $155,426
- **Award type:** 5
- **Project period:** 1983-04-01 → 1988-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129498

## Citation

> US National Institutes of Health, RePORTER application 3129498, STUDIES ON POLIOVIRUS USING INFECTIOUS VIRAL CDNA CLONES (5R01AI020017-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129498. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*