# INTERFERON PRODUCTION BY MOUSE BONE MARROW CELLS

> **NIH NIH R01** · UNIVERSITY OF TEXAS MED BR GALVESTON · 1988 · $107,946

## Abstract

Graft versus host disease (GVHD) is a major clinical problem complicating
bone marrow transplantation.  GVHD is dependent upon the presence of
T-lymphocytes in the donor tissue.  However, the actual role of these donor
T cells in the pathogenesis associated with GVHD is still unclear.  We have
postulated that interferon (IFN) may play an important role in the
pathogenesis associated with GVHD.  Many of the pathogenic sequelae
associated with GVHD can also be induced in vivo and in vitro by IFN.  We
have recently shown that mice undergoing acute GVHD have high levels of
serum IFN.  These results suggest that IFN may play a role in the
pathogenesis associated with GVHD.  My objective is to investigate the role
of IFN in the pathogenesis associated with acute GVHD.  We will
characterize the cells producing IFN and determine the type(s) of IFN
produced in different tissues obtained from mice undergoing acute GVHD.  We
will also characterize the T lymphocyte subpopulation(s) necessary for
inducing IFN production in acute GVHD.  These specific aims will be
addressed by measuring IFN from disrupted tissues obtained from mice at
various times during GVHD and/or by culturing cells obtained from these
mice in vitro and assessing these cultures for IFN production.  Using cDNAs
for IFNAlpha, IFNBeta, and IFNGamma, we will quantitate the amount of IFN
mRNA present in different tissues and/or cells by dot blot hybridization
and determine the tissue sites of IFN production by in situ hybridization.
The surface phenotype of cells producing IFN in vivo will be determined
with different well characterized monoclonal antisera using cell depletion
experiments or by in situ hybridization using cDNA probes for different
IFNs combined with immunocytochemistry.  We will determine if acute GVHD
can be altered by treating mice with antisera to different IFNs and/or by
treating mice with different lymphokines.  Mice transplanted with
allogeneic lymphoid tissue will be given monoclonal antisera to different
IFNs (IFNAlpha, IFNBeta, and/or IFNGamma) and GVHD assessed at various
times.  Transplanted mice will also be given one of the following:
IFNAlpha, IFNBeta, IFNGamma, IL3, and/or CSF-1 and GVHD assessed at various
times.  Lastly, we will measure and characterize the types of IFN present
in sera obtained from human bone marrow transplant patients undergoing
GVHD.  Findings from the studies proposed in this application will
contribute to our knowledge of IFN and its role in GVHD and to the field of
immunology and transplantation in general.

## Key facts

- **NIH application ID:** 3129539
- **Project number:** 5R01AI020055-05
- **Recipient organization:** UNIVERSITY OF TEXAS MED BR GALVESTON
- **SAM.gov UEI:** MSPWVMXXMN76
- **Principal Investigator:** GARY R. KLIMPEL
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $107,946
- **Award type:** 5
- **Project period:** 1983-05-01 → 1989-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129539

## Citation

> US National Institutes of Health, RePORTER application 3129539, INTERFERON PRODUCTION BY MOUSE BONE MARROW CELLS (5R01AI020055-05). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129539. Licensed CC0.

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