# REGULATION OF GRANULOCYTE SECRETION

> **NIH NIH R01** · UNIVERSITY OF MICHIGAN AT ANN ARBOR · 1989 · $237,130

## Abstract

Our studies are directed toward determining the biochemical
mechanisms involved in degranulation (secretion) of granulocytes
(PMN) which affect the adherent properties of PMN.  We propose
to focus on protein kinase C and its role in phosphorylation of
proteins which might be associated with the secretory process.
Protein kinase C will be purified from human PMN using high
performance liquid chromatography (HPLC).  Protein
phosphorylation patterns in whole cells, subcellular organelles,
and membrane fractions will be determined and correlated with
the PMN secretroy behavior.  We will purify an endogenous
inhibitor of PKC localized in specific granules of PMN.  The
inhibitor will be characterized with respect to its specific
intracellular localization, molecular weight, and activity as a
regulator of PKC.  To understand PMN secretion at a subcellular
level we will employ a model system suitable for quantitative
analysis which will allow us to examine in detail the
interrelationship between PKC binding to PMN membranes and
subsequent functional effects.  We will examine in detail some of
the components involved in promoting the adherence and fusion of
granules with membranes.  We will evaluate the specific
interaction of actin with granule membranes.  We will explore the
role of lipids, calmodulin, protein kinase C in promoting fusion of
granule membranes to phagosomes and to plasma membranes.  We
will categorize by 2-dimensional gel electrophoresis similarities
and differences between the plasma membrane and specific and
primary granules and secretory vesicles of PMN.  We will identify
intracellular organelle constituents such as lactoferrin and C3bi
on the plasma membrane of cells previously activated by
electroblotting.  We will examine the biochemical mechanisms
underlying the hyperresponsive behavior of specific granule
deficient PMNs which are unable to adhere normally to surfaces.
We will also examine by pulse-chase experiments the synthesis of
adhesive glycoproteins and their accumulation on the plasma
membrane of normal and deficient canine monocytes.

## Key facts

- **NIH application ID:** 3129576
- **Project number:** 5R01AI020065-08
- **Recipient organization:** UNIVERSITY OF MICHIGAN AT ANN ARBOR
- **SAM.gov UEI:** GNJ7BBP73WE9
- **Principal Investigator:** Laurence Alan Boxer
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $237,130
- **Award type:** 5
- **Project period:** 1982-08-01 → 1992-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129576

## Citation

> US National Institutes of Health, RePORTER application 3129576, REGULATION OF GRANULOCYTE SECRETION (5R01AI020065-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129576. Licensed CC0.

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