# EARLY EVENTS IN MAMMALIAN B-CELL DIFFERENTIATION

> **NIH NIH R01** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 1988 · $173,141

## Abstract

Formation of all blood cells initiates with common, multipotential
hemopoietic stem cells.  These are thought to give rise to committed
precursors which through a series of events expand and differentiate to
become functional B lymphocytes.  Our studies have been aimed at resolving
intermediate cell types in this pathway and probing mechanisms which may be
important for their regulation.  Methods were developed for obtaining stem
cell populations relatively free of their progeny cells and for using
functional and chromosomal markers to follow their fate after transfer to
irradiated and immunodeficient recipient mice.  Cell surface markers and
depletion techniques were used to distinguish different types of B cell
precursors and determine the time required experimentally for them to give
rise to funcional B cells.  Monoclonal antibodies were prepared to B
lineage antigens and used to enrich for B cell precursors from human and
murine bone marrow and from tissues of embryonic mice.  These have been
studied in detail and compared in terms of size, morphology,
ultrastructure, synthesis of immunoglobulin chains, and differentiative
capability in vivo and in vitro.  Additional studies in progress consider
their origins, migration after isolation and reinfusion, and configuration
of genes for light and heavy immunoglobulin chains.  Proposed research
includes plans for determining intrinsic differences in various B cell
precursors and ordering these in a differentiation sequence.  Culture
conditions have been established for observing at least the final stages in
B cell formation and emphasis will be placed on understanding how important
cellular interactions which take place in those circumstances are
mediated.  This will include a comprehensive series of studies directed
toward identifying macromolecules which affect B cell precursor
proliferation and maturation.  In addition to these experiments with normal
embryonic, neonatal, adult and aging animals, parallel investigations are
being conducted with established cell lines which are similar to murine
pre-B cells and an earlier type of cells which remain multiple options.

## Key facts

- **NIH application ID:** 3129596
- **Project number:** 5R01AI020069-06
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **SAM.gov UEI:** NGCNCJ1X6XA4
- **Principal Investigator:** Paul Wayne Kincade
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $173,141
- **Award type:** 5
- **Project period:** 1982-09-01 → 1988-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129596

## Citation

> US National Institutes of Health, RePORTER application 3129596, EARLY EVENTS IN MAMMALIAN B-CELL DIFFERENTIATION (5R01AI020069-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129596. Licensed CC0.

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