# ORGANIZATION AND EXPRESSION OF DNA TUMOR VIRUS GENOMES

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 1987 · $75,864

## Abstract

Our goal is to study the regulation of gene expression of DNA tumor virus
genomes at the molecular level and to use this knowledge to understand the
mechanisms of eukaryotic gene expression and virus-host cell interactions.
The objectives are to study the transcriptional regulation of adenovirus
type 5 early transcriptional region (EII) and to attempt to isolate and
characterize the host genes activated by the viral pre-early EIA gene.

Transcriptional control elements of the EIIA-late promoter will be
identified at the virus level using the linker scanning mutagenesis
approach.  Functional relationship of the transcriptional control elements
of the two DIIA (early and late) promoters and the genetic basis of their
differential regulation in viral lytic cycle will be studied using novel
mutant viruses.  Biochemical approaches such as gel assays for DNA-protein
complexes and in vivo assays involving exonuclease III will be employed to
identify transcription factors which may interact wih the transcriptional
control elements of the two EIIA promoters.

Utilizing molecular hybridization and gene cloning techniques, attempts
will be made to identify and isolate the host genes activated by the viral
pre-early EIA gene.

## Key facts

- **NIH application ID:** 3129642
- **Project number:** 5R01AI020156-05
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **SAM.gov UEI:** KG76WYENL5K1
- **Principal Investigator:** BAYAR THIMMAPAYA
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $75,864
- **Award type:** 5
- **Project period:** 1983-08-01 → 1991-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129642

## Citation

> US National Institutes of Health, RePORTER application 3129642, ORGANIZATION AND EXPRESSION OF DNA TUMOR VIRUS GENOMES (5R01AI020156-05). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3129642. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*