# MECHANISM OF ANTIGEN PRESENTATION BY MACROPHAGES

> **NIH NIH R01** · EMORY UNIVERSITY · 1990 · $128,634

## Abstract

The long range goal of these studies is to provide a detailed
understanding of the mechanism by which macrophages process
and present antigen to lymphocytes.  In this regard, attempts will
be made to understand the interactions of macrophages with the
facultative intracellular pathogen Listeria monocyogenes.  Assays
for macrophage-dependent T lymphocyte activation in vitro will
be used.  Antigenic determinants associated with the bacteria and
expressed by macrophages infected with the bacteria will be
detected with monoclonal antibodies and T cell lines and
hybridomas.  Detailed study of the antigenic structures of L
monocytogenes will be performed using such tools in combination
with molecular genetic techniques.  The molecular and cellular
mechanisms underlying the differential requirements for antigen
processing requirements with different forms of listerial antigens
will be studied.

Experiments will be performed to evaluate the effects of the
fungal polypeptide, cyclosporine, on macrophage-dependent in the
facilitation of clinical organ transplantation, its precise mode of
action remains unknown.  Future experiments will be performed
to clarify the mechanism of the apparent inhibition of antigen
presentation function by cyclosporine.

These studies should further the understanding of the mechanisms
by which intracellular pathogens Listeria and fungal products
(cyclosporine) regulate macrophage and lymphocyte function and
how immunity to microorganisms is generated and expressed.

## Key facts

- **NIH application ID:** 3129735
- **Project number:** 5R01AI020215-08
- **Recipient organization:** EMORY UNIVERSITY
- **SAM.gov UEI:** S352L5PJLMP8
- **Principal Investigator:** H KIRK ZIEGLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $128,634
- **Award type:** 5
- **Project period:** 1983-08-01 → 1992-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129735

## Citation

> US National Institutes of Health, RePORTER application 3129735, MECHANISM OF ANTIGEN PRESENTATION BY MACROPHAGES (5R01AI020215-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129735. Licensed CC0.

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