# HUMAN EOSINOPHILS--MECHANISMS OF FUNCTIONING

> **NIH NIH R01** · BETH ISRAEL DEACONESS MEDICAL CENTER · 1992 · $261,779

## Abstract

Eosinophilia is a frequent concomitant of allergic and certain related
immunologic responses.  To elucidate the role of the human eosinophilic
leukocyte in these responses, we will investigate several interrelated
aspects of the cellular functioning of human eosinophils.  The biochemical
and functional properties of selected, homogeneously purified eosinophil
proteins (including lysophospholipase, eosinophil peroxidase, and
histaminase) will be studied.  Eosinophil chemoattractant lymphokines
produced by antigen, mitogen and histamine stimulated human blood
mononuclear cells will be purified; the lymphokine producing cells will be
characterized and the effects of the purified lymphokines on eosinophil
migration, activation and poiesis will be assessed.  Utilizing electron
microscopy and sensitive radioimmunoassays developed for various granular
and extragranular human eosinophil proteins, the mechanisms of
extracellular secretion of these proteins from eosinophils will be
determined, and in conjunction with subcellular fractionation, the
intracellular compartmentalization of these constituents will be
determined.  Possible proteins synthesized by these stimulated eosinophils
will be investigated and their activities on other cell types (lymphocytes,
fibroblasts, granulocytes) assessed.  We hope to elucidate mechanisms of
eosinophil functioning in tissues during allergic and immunologic responses
and to define mechanisms whereby mast-cell released histamine, acting via
the elaboration of eosinophil-active lymphocyte products, may not only lead
to prolonged tissue eosinophilia but also to increased eosinophilpoiesis,
to activation of tissue eosinophils and possibly to collaboration of
eosinophils with other cells types.  With these studies, an integrated
understanding of the genesis, accumulation and functions of eosinophils at
sites of allergic responses may develop that will guide the rational
therapy of these allergic responses.

## Key facts

- **NIH application ID:** 3129770
- **Project number:** 5R01AI020241-09
- **Recipient organization:** BETH ISRAEL DEACONESS MEDICAL CENTER
- **SAM.gov UEI:** C1CPANL3EWK4
- **Principal Investigator:** PETER F WELLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $261,779
- **Award type:** 5
- **Project period:** 1983-07-01 → 1994-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129770

## Citation

> US National Institutes of Health, RePORTER application 3129770, HUMAN EOSINOPHILS--MECHANISMS OF FUNCTIONING (5R01AI020241-09). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129770. Licensed CC0.

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