# STUDY OF BIOLOGICALLY ACTIVE MICROBIAL METABOLITES

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1988 · $191,595

## Abstract

The research outlined in this proposal is aimed at elucidating the mode of
biosynthesis of a number of antibiotics.  Compounds to be studied are
pyrrolnitrin and the pyrrolomycins, the hydroaromatic antibiotics
ketomycin, dihydrophenylalanine and anticapsin, some benzoisochromane
quinone antibiotics, the macrocyclic antibiotics aplasmomycin, naphthomycin
and ansatrienine, several compounds containing biosynthetically unusual
structural units like asukamycin, manumycin, reductiomycin and acarbose,
and the modified peptide antibiotics nosiheptide and thiostreptone.
Biological structure modification based on biosynthetic information is also
envisioned with some of these compounds.  The methods to be used include
(a) feeding experiments with precursors labeled with radioactive isotopes,
but also to a large extent the use of 13C, 15N, 18O and deuterium labeled
compounds in conjunction with Fourier Transform NMR Spectroscopy, (b)
isolation of pathway intermediates from blocked mutants, (c) isolation of
enzymes carrying out some of these transformations and (d) synthesis and
use of stereospecifically labeled substrates to probe the stereochemistry
and mechanism of some of the biosynthetic reactions.

## Key facts

- **NIH application ID:** 3129833
- **Project number:** 7R01AI020264-06
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** HEINZ G FLOSS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $191,595
- **Award type:** 7
- **Project period:** 1987-12-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129833

## Citation

> US National Institutes of Health, RePORTER application 3129833, STUDY OF BIOLOGICALLY ACTIVE MICROBIAL METABOLITES (7R01AI020264-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3129833. Licensed CC0.

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