# STRUCTURE AND FUNCTION OF THE MAC-1 GENE

> **NIH NIH R01** · DANA-FARBER CANCER INST · 1989 · $146,356

## Abstract

The objective of the project is to obtain full length genomic DNA
clones and cDNA clones for both the alpha and beta-chains of the
functionally important cell surface antigen, MAC-1, and to
characterize these clones in detail.  The complete DNA sequence
of the cDNA clones will be determined and from this the protein
sequence of MAC-1 deduced.  Extensive studies on the
relationship between structure and function of the protein will be
undertaken using a variety of mutagenesis protocols.  Mutants in
the protein will be assayed for function using a very efficient
protocol based upon an easily rescued retrovirus vector.  The
promoters for both MAC-1 genes will be mapped on the genomic
clones and further characterized by genetic analysis.  Changes in
the level of methylation of the gene and of the DNAse reactivity
of the gene's chromatin will be studied as a function of
macrophage differentiation.  Proteins which bind to the genes
control region will be characterized by Crothers' gel analysis.

## Key facts

- **NIH application ID:** 3129882
- **Project number:** 5R01AI020316-06
- **Recipient organization:** DANA-FARBER CANCER INST
- **SAM.gov UEI:** DPMGH9MG1X67
- **Principal Investigator:** THOMAS M ROBERTS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $146,356
- **Award type:** 5
- **Project period:** 1983-08-01 → 1990-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3129882

## Citation

> US National Institutes of Health, RePORTER application 3129882, STRUCTURE AND FUNCTION OF THE MAC-1 GENE (5R01AI020316-06). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3129882. Licensed CC0.

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