# UTILIZATION OF EPIDERMAL CELL MODEL FROM HUMAN SKIN

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1987 · $180,363

## Abstract

HUMAN SKIN - NUDE MOUSE MODEL.  We have established a model for long-term
cultivation of adult human skin on the nude athymic mouse.  Psoriatic skin can
be grown for periods of several months and retain the essential features of
psoriasis.  Epidermal kinetics studies confirm a hyperproliferative state in
these grafts.  Recombinant studies of normal and psoriatic epidermis and dermis
have localized the site of the pathologic defect in psoriasis to both epidermis
and dermis.  Studies are also underway utilizing the human skin-nude mouse
system to study conservation of regional epidermal specificities and the effect
of non-dermal connective tissue on epidermal maintenance.  Epidermal kinetic
studies are also underway on normal adult human skin to measure the percentage
of cycling cells (growth fraction).

Formation and Function of Structures at the Epidermal-Dermal Junction in Normal
and Diseased Human Skin.  Studies are underway to selectively isolate components
of the epidermal-dermal junction, particularly anchoring fibrils.  Crude
"anchoring fibril" enriched extracts have been obtained from human skin.  A
monoclonal antibody has been prepared that reacts with a normal human skin
basement membrane antigen by immunofluorescent studies.  On
immunoelectronmicroscopy, monoclonal basement membrane zone antibody localized
to the sub-basal lamina area, particularly associated with anchoring fibrils.
Plans are to further document this association and to use this antibody as a
tool to selectively isolate anchoring fibrils from crude "anchoring fibril
enriched" preparations.

## Key facts

- **NIH application ID:** 3154706
- **Project number:** 5R01AR010546-22
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** ROBERT A BRIGGAMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $180,363
- **Award type:** 5
- **Project period:** 1977-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154706

## Citation

> US National Institutes of Health, RePORTER application 3154706, UTILIZATION OF EPIDERMAL CELL MODEL FROM HUMAN SKIN (5R01AR010546-22). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3154706. Licensed CC0.

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