# STUDIES ON THE RENAL DISEASE OF SYSTEMIC LUPUS

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1990 · $310,846

## Abstract

The work will characterize pathogenic mechanisms of disease in patients
with systemic lupus erythematosus (SLE).  New knowledge will be sought on
how immune complexes will deposit in tissues, using experimental animals.

Previous studies have shown that patients with SLE have IgG in circulation
that binds to Ckq in solid phase assays.  Immunochemical techniques
techniques will be used to determine if these molecules bind to Clq as
antibodies or as small immune complexes.  The isoelectric points of
antibodies in large immune complexes of patients with SLE will be
determined by isoelectric focusing and these findings will be related to
the nature of renal disease of these patients.  These studies will
determine if charge-charge interactions contribute to glomerular deposition
of immune complexes in patients with SLE.  The nature and specificity of
antibodies and antigens will be determined in serum immune complexes of
patients with SLE.  After evaluation of elution and detection methods with
material from mouse models, the nature of antibodies and their specificity
in immune material from mouse models, the nature of antibodies and their
specificity in immune deposits at the dermal-epidermal junction of patients
with SLE will be determined.

In experimental animals mechanisms of immune complex deposition in
glomeruli will be studied.  The deposition in glomeruli of immune complexes
with complement components will be tested.  The contribution of positively
charged regions of antigen molecules to deposition in glomeruli by
charge-charge interaction will be investigated.  The contribution of
hypertension to mesangial deposition of immune complexes will be
evaluated.  The role of concentration dependent polymerization of
macromolecules in deposition in glomerular mesangium will be examined with
native and modified macromolecules for which polymerization will be
characterized.

## Key facts

- **NIH application ID:** 3154750
- **Project number:** 5R01AR011476-23
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** MART MANNIK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1990
- **Award amount:** $310,846
- **Award type:** 5
- **Project period:** 1976-06-25 → 1991-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154750

## Citation

> US National Institutes of Health, RePORTER application 3154750, STUDIES ON THE RENAL DISEASE OF SYSTEMIC LUPUS (5R01AR011476-23). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3154750. Licensed CC0.

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