# STUDIES OF AUTOANTIBODIES AND IMMUNOGLOBULINS

> **NIH NIH R01** · UNIVERSITY OF NEW MEXICO · 1987 · $174,836

## Abstract

This proposal will extend work in three main areas:  1) pathogenesis of
acute rheumatic fever and its possible relationship to B-cell alloantigens
defined by two recently-developed mouse monoclonal antibodies 883 and 256;
2) studies of antiidiotypic control mechanisms influencing anti-ds-DNA
antibody production during the course of systemic lupus erythematosus
(SLE); and 3) further investigations concerning immunochemical specificity
and possible physiologic significance of anti-lymphocyte antibodies in
patients with SLE.  The work directed at possible B-cell alloantigen
control of host immune response to group A streptococcal antigens will
attempt to provide better immunochemical definition of the B-cell surface
components reacting with the 883 and 256 reagents.  In addition,
comparative in vitro assays are planned using various streptococcal and
control antigens in experiments using peripheral blood and mononuclear cell
stimulation and macrophage pulsing with 883/256 (+) and (-) donors.
Studies of anti-idiotypic control mechanisms in SLE will utilize monoclonal
murine antibodies produced against a panel of isolated SLE anti-ds-DNA
antibodies as well as radioimmunoassay and ELISA techniques to define
auto-anti-DNA idiotypic reactions.  The possibility that antibodies showing
anti-F(ab')2 specificity may be representative of a broad pool of
anti-idiotypes some of which are enriched for antids-DNA anti-idiotypic
reactivity will be sequentially explored using affinity column purified
anti-DNA and anti-F(ab')2 antibodies and ELISA assay techniques.  Finally,
specific studies of lymphocyte glycoproteins reacting with anti-lymphocyte
antibodies from SLE will be performed using lymphocyte cell membrane
proteins separated by SDS PAGE gels and transferred to nitrocellulose
paper.  Further definition of the mechanisms whereby IgG antibodies from
SLE serum are capable of penetrating living cells will be performed using
sequential electronmicroscope monitoring.

## Key facts

- **NIH application ID:** 3154775
- **Project number:** 5R01AR013690-19
- **Recipient organization:** UNIVERSITY OF NEW MEXICO
- **SAM.gov UEI:** F6XLTRUQJEN4
- **Principal Investigator:** RALPH C WILLIAMS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $174,836
- **Award type:** 5
- **Project period:** 1976-05-01 → 1988-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154775

## Citation

> US National Institutes of Health, RePORTER application 3154775, STUDIES OF AUTOANTIBODIES AND IMMUNOGLOBULINS (5R01AR013690-19). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3154775. Licensed CC0.

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