# PSORIASIS AND ICHTHYOSIS IN VIVO AND IN CELL CULTURE

> **NIH NIH R01** · YALE UNIVERSITY · 1989 · $193,820

## Abstract

A.  Examine the production of several cytokines by normal,
malignant and transformed human and murine keratinocytes.
Specifically the synthesis, translocation, membrane insertion and
secretion of two cytokines will be examined.  These are ETAF (IL-
1-like activity) and KTGF (which has IL-2 activity).  The control
of secretion of these two cytokines by UVA, UVB, temperature
elevation and LPS will be compared.  It will be determine whether
the epidermal cytokines are released coordinately or whether they
are under separate control.  The regulation of ETAF release by
glucocorticosteroids (GCS) will be examined to determine where
in the process of cytokine synthesis and release, the GCS
function.  ETAF (IL-1) and KTGF (IL-2) are assayed with D-10 and
HT-2 responder helper T lymphocytes respectively.

B.  The production of cytokines by individuals with disorders of
epidermal proliferation will be examined in vivo, in tissue slice
(organ culture) and in tissue culture.  The responsiveness of
cytokine production to GCS, temperature elevation and
ultraviolet light will be measured.

C.  The influence of cytokines derived from keratinocytes and
lymphocytes on keratinocyte growth and differentiation will be
examined using several different markers of differentiation
including plasminogen activator, cross-linked envelope formation,
the pattern of keratin synthesis and the presence of pemphigus
vulgaris antigen.  Cytokines will be isolated by means of HPLC
using both a sizing column (TSK-125) and reversed phase
chromatography (HI PORE RP 318).  Cytokines will be isolated
from normal and transformed keratinocytes as well as WeHi, a
source of colony stimulating factor (IL-3).

D.  Lymphocytes enter the epidermis in many disorders.  The
chemotactic effect of keratinocyte-conditioned media for helper
and suppressor T-cells will be studied and using fractions purified
by HPLC, the cytokine(s) responsible for chemotaxis will be
identified.  Chemotaxis by keratinocyte cytokines results in the
appearance in the epidermis of lymphocytes the leading front
analysis in micropore filter assays in Boyden chambers and time-
lapse photography using sepharose beads impregnated with
cytokines.

## Key facts

- **NIH application ID:** 3154803
- **Project number:** 5R01AR013929-19
- **Recipient organization:** YALE UNIVERSITY
- **SAM.gov UEI:** FL6GV84CKN57
- **Principal Investigator:** JOSEPH S MC GUIRE
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $193,820
- **Award type:** 5
- **Project period:** 1978-09-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154803

## Citation

> US National Institutes of Health, RePORTER application 3154803, PSORIASIS AND ICHTHYOSIS IN VIVO AND IN CELL CULTURE (5R01AR013929-19). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3154803. Licensed CC0.

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