# NUCLEIC ACID AND PROTEIN METABOLISM IN CARTILAGE

> **NIH NIH R01** · MASSACHUSETTS GENERAL HOSPITAL · 1987 · $274,018

## Abstract

The purpose of this research proposal is to continue the study of normal
and pathological articular and epiphyseal cartilage with special attention
to the metabolims of the tissue in health and a variety of disease states,
especially human osteoarthritis.  The studies to be performed include a
continued study of DNA replicative activity in normal and pathological
cartilages with special emphasis on the use of flow cytometry; a continued
definition of the products and rates of synthesis of normal and pathologic
cartilages utilizing a variety of biochemical and metabolic techniques
including the cell-free systems; a continued study of a variety of normal
and pathological control mechanisms of the metabolic (both anabolic and
catabolic) activities of normal and abnormal cartilages; a search for
intracellular and cell membrane markers of disease states in normal and
pathologic cartilages, chiefly by use of the flow cytometer; and continued
attempts at isolation, identification, purification and determination of
optimal conditions, substrate, cofactors and inhibitors for degradative
enzymes in normal and diseased articular and epiphyseal cartilages.

## Key facts

- **NIH application ID:** 3154891
- **Project number:** 5R01AR016265-16
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **SAM.gov UEI:** FLJ7DQKLL226
- **Principal Investigator:** HENRY J MANKIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $274,018
- **Award type:** 5
- **Project period:** 1977-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154891

## Citation

> US National Institutes of Health, RePORTER application 3154891, NUCLEIC ACID AND PROTEIN METABOLISM IN CARTILAGE (5R01AR016265-16). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3154891. Licensed CC0.

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