# BIOLOGY AND CHEMISTRY OF PROCOLLAGEN IN CELL CULTURE

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 1989 · $148,303

## Abstract

This is a competing continuation request for another five years of research
support.  The major focus of the proposed studies is upon the structure,
function and biological expression of macromolecules of the extracellular
matrix, examining the protein and carbohydrate structure of laminin and the
gene structure for the core protein of chondroitin sulfate proteoglycan.
Laminin is isolated from the EHS tumor of mice and the specific chondroitin
sulfate core protein gene under study is that of chicken embryo sternal
cartilage.

In the case of laminin, the objectives are to characterize the molecular
anatomy of that glycoprotein, describing the relationships of the
polypeptide subunits in the entire molecule as well as the relationships of
the oligosaccharides to the polypeptides.  The subunits will be isolated
and their compositions and structures determined, including the location
and structures of the major carbohydrate substituents.  The interactions
between the subunits in the molecule will be examined by selective
dissociation of disulfide linkages and by immuno-electron microscopy, using
specific antibodies to each subunit.

In the case of the gene which encodes for core protein, the cDNA structure
will be determined, and genomic clones will be isolated from a library of
chicken DNA, followed by determination of the sequence of the genomic DNA.
Parallel studies of core protein mRNA expression in a well-characterized
differentiating system, the chick embryo limb bud, will be done as well as
determination of the copy number of the gene in chicken tissues.  A human
genomic library will also be screened with either the chicken cDNA probe or
genomic chicken DNA.

These two projects encompass the scientific disciplines of biochemistry and
molecular biology, utilizing contemporary methods to discern protein
structure, carbohydrate structure, and gene structure.  The
health-relatedness of the research applies to the general area of acquired
and hereditary connective tissue diseases.

## Key facts

- **NIH application ID:** 3154943
- **Project number:** 5R01AR017220-17
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **SAM.gov UEI:** H6D6JMXJXDE6
- **Principal Investigator:** MARVIN L TANZER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $148,303
- **Award type:** 5
- **Project period:** 1976-09-01 → 1991-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154943

## Citation

> US National Institutes of Health, RePORTER application 3154943, BIOLOGY AND CHEMISTRY OF PROCOLLAGEN IN CELL CULTURE (5R01AR017220-17). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3154943. Licensed CC0.

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