# SARCOPLASMIC RETICULUM FUNCTION AND REASSEMBLY

> **NIH NIH R01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 1987 · $183,897

## Abstract

The objective of this proposal is to determine the molecular properties of
a Ca2+ release channel in sarcoplasmic reticulum of muscle.  The Ca2+
release channel in isolated sarcoplasmic reticulm vesicles is capable of
mediating Ca2+ fluxes with a physiological rate suggesting that it plays an
important role in the process of excitation-contraction coupling in
muscle.  Sedimentation and equilibrium centrifugation techniques will be
used to isolate the following subfractions from rabbit skeletal muscle:
"heavy" sarcoplasmic reticulum vesicles containing the CA2+ release
channel, "light" sarcoplasmic reticulum vesicles lacking the Ca2+ release
channel, surface membranes (T-system and plasmalemma), and triads
(sarcoplasmic reticulum/T-system junctional complexes).  Monoclonal
antibodies to enriched triad fractions and sarcoplasmic reticulum vesicles
containing the Ca2+ release channel will be generated in order to localize
and establish the function of specific membrane components in
excitation-contraction coupling.  The mechanism of regulation of the Ca2+
release channel by Ca2+, Mg2+ adenine nucleotides, calmodulin, and other
factors will be determined.  The C2+ release channel of sarcoplasmic
reticulum will be incorporated into planar lipid bilayers so that single
channel conductance, ion selectivity and voltage-dependence, as well as the
kinetics of channel opening and closing can be investigated.  Ca2+ efflux
from sarcoplasmic reticulum vesicles will be measured, using rapid quench
techniques.  T-system depolarization-induced Ca2+ release from the
sarcoplasmic reticulum compartment will be measured using isolated triads
in order to determine how a muscle action potential at the cell surface
induces release of Ca2+, and thereby muscle contraction.  In addition,
affinity labeling techniques and/or monoclonal antibodies will be used to
identify, purify and reconstitute the channel protein(s).

## Key facts

- **NIH application ID:** 3154994
- **Project number:** 5R01AR018687-12
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **SAM.gov UEI:** D3LHU66KBLD5
- **Principal Investigator:** GERHARD W MEISSNER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $183,897
- **Award type:** 5
- **Project period:** 1976-05-01 → 1990-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3154994

## Citation

> US National Institutes of Health, RePORTER application 3154994, SARCOPLASMIC RETICULUM FUNCTION AND REASSEMBLY (5R01AR018687-12). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3154994. Licensed CC0.

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