# A VOLTAGE-GATED K+ CHANNEL OF SARCOPLASMIC RETICULUM

> **NIH NIH R01** · BRANDEIS UNIVERSITY · 1987 · $161,995

## Abstract

Ion conductances of isolated sarcoplasmic reticulum (SR) from vertebrate
skeletal muscle will be studied to address two types of questions:  (1) how
are the electrical properties of the SR membrane related to contractile
activation in muscle, and (2) what are the basic mechanisms by which the K+
channel of SR operates.  Two broad methods will be used:  the fusion of
isolated SR vesicles into planar phospholipid bilayer membranes, followed
by electrical measurement of ionic currents through single channels, and
the direct observation of rapid fluxes of ions into SR vesicles via a
stopped-flow fluorescence quenching method with millisecond time resolution.

K+ channel properties in planar bilayers will be examined in detail, in
particular those giving indications of multiple ion interactions within the
channel protein.  In addition, the SR conductances for anions will be
characterized.  Specific inhibitors of SR conductances will be sought, both
by snythesizing blocker compounds and by searching for endogenous
regulators in muscle.

## Key facts

- **NIH application ID:** 3155077
- **Project number:** 5R01AR019826-11
- **Recipient organization:** BRANDEIS UNIVERSITY
- **SAM.gov UEI:** MXLZGAMFEKN5
- **Principal Investigator:** Christopher Miller
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $161,995
- **Award type:** 5
- **Project period:** 1977-04-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155077

## Citation

> US National Institutes of Health, RePORTER application 3155077, A VOLTAGE-GATED K+ CHANNEL OF SARCOPLASMIC RETICULUM (5R01AR019826-11). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3155077. Licensed CC0.

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