# ADENINE NUCLEOTIDE METABOLISM IN SKELETAL MUSCLE

> **NIH NIH R01** · UPSTATE MEDICAL UNIVERSITY · 1988 · $127,416

## Abstract

The general purpose of this proposal is to evaluate aspects of adenine
nucleotide metabolism in skeletal muscle.  The influence of exercise, the
differences between skeletal muscle fiber types, the influence of exercise
training and the physiological significance of certain metabolic pathways
will be assessed.  Although muscle contractions establish a high rate of
ATP hydrolysis, the concomitant acceleration in the rate of ADP
phosphorylation tends to limit the decrease in ATP concentration.  During
intense exercise conditions, however, a reduction of the total adenine
nucleotide pool (TAN = ATP + ADP + AMP) can occur.  This is due to
degradative reactions leading to IMP, nucleoside and purine production.
The quantity and nature of the products are probably not the same in the
different skeletal muscle fiber types.  The extent of adenine nucleotide
degradation during muscle stimulation, the differences between muscle fiber
types and the dependence on the metabolic stress will be determined using
an isolated perfused rat hindlimb preparation.  Assays and purification of
adenine nucleotides, purine nucleosides and purines will be performed using
high performance liquid chromatography.  Recovery of TAN following exercise
via de novo synthesis and/or purine salvage pathways, factors that
influence the synthesis pathways and their interaction will be assessed in
the different muscle fiber types.  The impact of muscle adaptations,
induced by exercise training, on the degradation/synthesis processes will
be assessed.  The activity of key pathway enzymes will be determined under
optimal conditions in vitro and related to the degradation and synthesis
processes determined previously for each fiber type.  The metabolic and
functional consequences of AMP deaminase will be determined by inhibiting
IMP production during moderate to intense exercise.  These unique studies
will provide fundamental information important for the understanding of
adenine nucleotide metabolism in the various fiber types of normal muscle.
This information, in turn, should greatly increase our understanding of
abnormalities in adenine nucleotide metabolism found in certain myopathies,
and the potential consequences of related muscle enzyme deficiencies.

## Key facts

- **NIH application ID:** 3155195
- **Project number:** 5R01AR021617-11
- **Recipient organization:** UPSTATE MEDICAL UNIVERSITY
- **SAM.gov UEI:** HYN3WD58HNN7
- **Principal Investigator:** Ronald L Terjung
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $127,416
- **Award type:** 5
- **Project period:** 1977-08-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155195

## Citation

> US National Institutes of Health, RePORTER application 3155195, ADENINE NUCLEOTIDE METABOLISM IN SKELETAL MUSCLE (5R01AR021617-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155195. Licensed CC0.

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