# DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1991 · $271,062

## Abstract

The long term objective of the proposed research is to elucidate the
structural and chemical basis of muscle contraction.  This objective will
be pursued by working on three related research projects aimed at the
clarification of: (1) the regulation of muscle contraction; (2)
function-structure relationships on actin, myosin, and actomyosin
interface; and (3) the assembly of myofibrilar structures.
     (1)The specific aims of the regulation project are: (i) to test the
hypothesis that the N-terminal segment of actin is involved in the
regulation of contraction in different muscles; (ii) to clarify the
interaction of caldesmon with actin; and (iii) to test under
physiological conditions the steric block and catalytic mechanisms for
regulation of muscle contraction.
     (2)The goals of the studies on actin, myosin, and actomyosin
interface are: (i) to clarify the role of selected sites on actin and
myosin in the strong (-ATP) and weak (+ATP) actomyosin binding; and (ii)
to determine the role of the SHI peptide, selected sites on actin and
myosin, and dynamic motions in actin in the contractile process.
     The first two projects will involve immunochemical, enzymological,
chemical modification, in vitro motility, fluorescence and sedimentation
experiments with peptide antibodies, modified and unmodified proteins,
actin mutants, myosin and actin filaments, myofibrils, and muscle fibers.
     (3)The specific aims of the assembly project are: (i) to clarify the
role of regulatory factors in the nucleation, growth, and stability of
myosin filaments; (ii) to characterize solution properties of titin; and
(iii) to examine the formation of myosin filaments in myofibrils by using
myosin from skeletal muscle hybridized with light chain-2 from smooth
muscle myosin.  These studies will employ hydrodynamic, light scattering,
electron microscopy, and spectroscopic techniques.
     The research proposed in this application will result in an improved
understanding of contractile processes in muscle and non-muscle cells.

## Key facts

- **NIH application ID:** 3155224
- **Project number:** 2R01AR022031-14
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** EMIL REISLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $271,062
- **Award type:** 2
- **Project period:** 1978-07-01 → 1995-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155224

## Citation

> US National Institutes of Health, RePORTER application 3155224, DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE (2R01AR022031-14). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3155224. Licensed CC0.

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