# DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 1988 · $205,404

## Abstract

The long term objective of the proposed research is to elucidate the
structural and chemical events in myosin, which lead to the contraction of
muscle.  This objective will be pursued by undertaking three inter-related
research projects aimed at the clarification of (1) the structure and
interaction of myosin heads with actin at the molecular level; (2) the
function and structural changes in myosin filaments in intact myofibrils
and muscle fibers; (3) the assembly of myosin molecules into functional
filament units, and the properties of such filaments.

(1) The specific aims of the proposed studies on the myosin head are:  i)
to clarify the role of specific sites on myosin and actin in actomyosin
binding; ii) to derive new structural information and define
structure-function relationships on the myosin head.  These studies will
involve diverse biochemical and biophysical methods including site specific
chemical modifications, chemical cross-linkings, selective proteolysis,
immunochemical techniques, electron microscopy, and fluorescence techniques.

(2) The specific aims of the studies on the function and structural changes
in myosin filaments are:  i) to determine the binding of myosin to actin in
the presence of ATP by using synthetic filament systems, myofibrils, and
muscle fibers; ii) to clarify the effect of nucleotide analogue (AMPPNP) on
the conformation of myosin in myofibrils and muscle fibers; iii) to explore
contraction and stress-induced changes in myosin.  This work will utilize
proteolytic methods, chemical cross-linkings, and chemical modification
reactions to test different regions on myosin, and to measure its binding
to actin.

(3) The specific aims of the work on the assembly of myosin into filaments
are: i) to characterize the nucleation step in filament assembly; ii) to
clarify the factors involved in the regulation of filament nucleation and
growth.  These studies will employ hydrodynamic, light scattering, electron
microscopy, and spectroscopic techniques.

The research proposed in this application will bring an improved
understanding of contractile processes in muscle and non-muscle cells.

## Key facts

- **NIH application ID:** 3155226
- **Project number:** 5R01AR022031-11
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **SAM.gov UEI:** RN64EPNH8JC6
- **Principal Investigator:** EMIL REISLER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $205,404
- **Award type:** 5
- **Project period:** 1978-07-01 → 1991-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155226

## Citation

> US National Institutes of Health, RePORTER application 3155226, DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE (5R01AR022031-11). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155226. Licensed CC0.

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