# BONE CELL CALCIUM REGULATION

> **NIH NIH R01** · STATE UNIVERSITY OF NEW YORK AT BUFFALO · 1988 · $121,429

## Abstract

The long term objective of this study is to understand the nature
of bone cell calcium regulation.  The endogenous and exogenous
factors that control this parameter will be assessed and the
potential interrelationships between these factors and calcium in
the regulation of bone metabolism will be evaluated.  The effects
of parathyroid hormone (PTH), prostaglandin E2 (PGE2), epidermal
growth factor (EGF) and interleukin -I(IL-l) will be studied in
normal rat osteoblastic cells and the osteosarcoma lines - UMR-106
and SaOS-2.  The interrelationships between putative effects of
these agents on calcium and phosphoinositide metabolism will also
be studied by investigating the regulation of this phospholipid in
the bone cells and by studying the effects of inositol phosphates
on calcium mobilization in these isolated cell systems.
Interrelationships between arachidonic and cytosolic calcium will
also be evaluated and the effects of arachidonic acid on
intracellular calcium mobilization will also be studied.  The role
of protein kinase C in the regulation of osteoblastic calcium
metabolism will also be studied using inhibitors and activators of
this enzyme.  Studies on protein, DNA synthesis and plasminogen
activator will also be conducted in an attempt to make correlations
with changes in cytosolic calcium.  Arachidonic acid metabolism
will also be studied in the various cell systems to better
understand the interrelationship between cyclooxygenase products
and cytosolic calcium.  Cytosolic calcium levels will be measured
using the Fura 2 fluorescent assay with cells in suspension and
attached to coverslips.  Phosphoinositol metabolism will be studied
with thin layer and ion exchange chromatography and high
performance liquid chromatography.  Arachidonic acid metabolism
will also be studied by high performance liquid chromatography.
These studies should lead to a better understanding of the nature
of bone cell metabolism and aid in the development of therapy for
systemic and localized bone metabolic disorders.

## Key facts

- **NIH application ID:** 3155289
- **Project number:** 2R01AR025271-10A2
- **Recipient organization:** STATE UNIVERSITY OF NEW YORK AT BUFFALO
- **SAM.gov UEI:** LMCJKRFW5R81
- **Principal Investigator:** ROSEMARY Mary DZIAK
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $121,429
- **Award type:** 2
- **Project period:** 1988-04-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155289

## Citation

> US National Institutes of Health, RePORTER application 3155289, BONE CELL CALCIUM REGULATION (2R01AR025271-10A2). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155289. Licensed CC0.

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