# STRUCTURE AND IMMUNOLOGY OF CARTILAGE PROTEOGLYCAN

> **NIH NIH R01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 1989 · $230,426

## Abstract

Proteoglycan is a basic structural element of cartilage, critical in its
physiological function and centrally involved in pathological processes
causing cartilage destruction in various forms of arthritis.  This
application requests continued funding for studies of the primary structure
and immunological properties of the proteoglycan of bovine nasal
cartilage.  Methods have been developed for the identification and
isolation of the tryptic fragments of bovine nasal cartilage proteoglycan
and several major fragments have been obtained in fractions sufficiently
homogeneous to allow their biochemical and immunological characterization
as well as N-terminal amino acid sequence determination.  Under the present
proposal, additional proteoglycan fragments will be isolated by gel
chromatography or HPLC following the removal of their carbohydrate
constituents by chemical or enzymatic means.  Individual tryptic fragments
will be enzymatically or chemically cleaved, the subfragments isolated and
characterized in terms of their biochemical and immunological properties,
and their amino acid sequences determined.  Analysis of these isolated
tryptic subfragments of proteoglycan, and of selected overlapping sequences
from proteoglycan fragments produced by alternate means, should allow
reconstruction of the primary structure of all or most of the intact
molecule.  In addition, it is proposed to utilize hybridoma technology and
a solid-phase immunoassay to develop a series of monoclonal antibodies
directed against specific structurally defined antigenic determinants
localized to individual isolated proteoglycan subfragments.  The
appropriate monoclonal antibodies can be used to elucidate the structure of
proteoglycans in other tissues and species, and to approach the study of
the molecular biology and genetics of proteoglycans.  Better knowledge of
the structure, immunology and molecular biology of cartilage proteoglycan
can lead to a better understanding of what happens to cartilage on a
molecular level in the course of arthritis and hopefully point to more
effective therapeutic modalities.

## Key facts

- **NIH application ID:** 3155342
- **Project number:** 5R01AR025711-10
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** HAROLD D KEISER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1989
- **Award amount:** $230,426
- **Award type:** 5
- **Project period:** 1979-07-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155342

## Citation

> US National Institutes of Health, RePORTER application 3155342, STRUCTURE AND IMMUNOLOGY OF CARTILAGE PROTEOGLYCAN (5R01AR025711-10). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155342. Licensed CC0.

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