# CELL-SUBSTRATE INTERACTIONS IN EPIDERMAL CELL MIGRATION

> **NIH NIH R01** · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · 1987 · $113,860

## Abstract

The major objective of these studies is to increase our understanding of
how epidermal cells interact with the extracellular matrix (ECM) during
wound closure.  Thus, the behavior of adult newt (Notophthalmus
viridescens) epidermal cells in wounded limbs will be analyzed as the cells
migrate over plastic or nitrocellulose implants coated with collagen,
laminin, fibrinogen (FGN), or fibronectin (FN).  To learn more about the
molecular features that epidermal cells recognize in FGN and FN,
proteolytic fragments of each molecule will be tested as migration
substrates and/or soluble inhibitors of migration over the intact
molecule.  In the case of FN, monoclonal antibodies will also be tested as
inhibitors of migration over the intact molecule to determine if epidermal
cells utilize the fibroblast binding site of FN.  To begin characterizing
the cell surface receptors which might be involved in recognition of ECM
molecules, binding studies will be conducted using latex beads coated with
various ECM proteins to determine if the receptors on the upper surface of
migrating cells vary in relation to the substrate under the cells.  Lectins
and glycosidases will be tested as migration substrates to determine if
bonds between cell surface saccharides and the substrate could be involved
in migration.  In addition, adhesion assays will be conducted using
dissociated cells in an attempt to demonstrate interaction of epidermal
cells with migration-promoting ECM proteins in a more defined environment
than a wound.  To analyze certain consequences of substrate recognition,
immunohistochemical techniques will be used to study the expression of
basement membrane zone proteins as epidermal cells migrate over implants
coated with various ECM molecules.  Finally, experiments will be conducted
to determine if epidermal cells lose their dependence on the pre-existing
ECM during migration.

## Key facts

- **NIH application ID:** 3155591
- **Project number:** 5R01AR027940-06
- **Recipient organization:** UNIVERSITY OF TENNESSEE HEALTH SCI CTR
- **SAM.gov UEI:** X1M1PN3KG3E7
- **Principal Investigator:** DONALD J DONALDSON
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $113,860
- **Award type:** 5
- **Project period:** 1982-04-01 → 1990-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155591

## Citation

> US National Institutes of Health, RePORTER application 3155591, CELL-SUBSTRATE INTERACTIONS IN EPIDERMAL CELL MIGRATION (5R01AR027940-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155591. Licensed CC0.

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