# CONTROL OF DIFFERENTIATION IN THE MYOGENIC LINEAGE

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 1987 · $143,090

## Abstract

Long-Term Objectives:

Determination of the programmed and environmental controls operating in the
differentiation of normal skeletal muscle cells.  Applicability of
regeneration, aging.

Specific Aims:

Testing of predictions and general validity of a lineage model of
myogenesis both in vitro and in vivo.  In particular:

1. Isolation and characterization of the myogenic stem cell.

a. Determination of which proteins or active genes are unique to this cell.

b. Determination of the environmental controls that affect the types of
progeny produced by this cell.

c. Determination of whether this cell decides what types of proteins are to
be synthesized at the end of the lineage.

d. Determination of the relationship of this cell to muscle fibroblasts.

2. Testing the lineage model in vivo in the chick embryo.

3. Testing the model in other species.

4. Identification of the lineage stage-specific markets (genetic and/or
protein) and markers for non-myogenic cells.

5. Determination of chromatin structural changes as cells traverse the
lineage.

Methodology:

Tissue culture, protein purification, polyclonal and monlclonal antibody
production and characterization, autoradiography, cell fractionation by
density gradient centrifugation, cloning of chick embryo precursor cell
genes (cDNA's), immunocytochemistry.

## Key facts

- **NIH application ID:** 3155616
- **Project number:** 5R01AR028154-06
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **SAM.gov UEI:** HD1WMN6945W6
- **Principal Investigator:** MARK A NAMEROFF
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $143,090
- **Award type:** 5
- **Project period:** 1982-04-01 → 1989-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155616

## Citation

> US National Institutes of Health, RePORTER application 3155616, CONTROL OF DIFFERENTIATION IN THE MYOGENIC LINEAGE (5R01AR028154-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155616. Licensed CC0.

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