# STRUCTURE-FUNCTION RELATIONS IN MYOSIN

> **NIH NIH R01** · BOSTON BIOMEDICAL RESEARCH INSTITUTE · 1988 · $153,686

## Abstract

Detailed information about the interface between actin and myosin is needed
for a better understanding of various aspects of the actin-myosin
interaction underlying the crossbridge cycle of muscle contraction.  We
also need information on the interaction between myosin heavy chains and
light chains to understand the function of light chains and the topography
of myosin S-1.  In view of the importance attributed to the so-called hinge
regions in the in vivo motion of myosin cross-bridges, information on the
conformation of various portion of the rod is desirable.  Thus the
application will focus on the following specific aims: 1). Identification
of the interface regions using a number of bifunctional crosslinkers under
a variety of conditions.  2). Completion of the determination of the
primary structure of myosin rod.  3). Exploration of the conformation of
different portions of myosin rod by examining the ability of sulfhydryls to
form disulfide bonds and the susceptibility of these regions to proteolytic
enzymes under various conditions.  4). Localization of light chains in
relation to heavy chains by crosslinkling experiments.  5). Crosslinking
studies of LMM and identification of the interaction regions with a view to
obtaining information relevant to filament formation.  The knowledge of
normal muscle will serve as a reference and may eventually prove useful in
the diagnosis, treatment, and prevention of disease involving muscle tissue
and the cardiovascular system.

## Key facts

- **NIH application ID:** 3155641
- **Project number:** 5R01AR028401-07
- **Recipient organization:** BOSTON BIOMEDICAL RESEARCH INSTITUTE
- **Principal Investigator:** RENNE C LU
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $153,686
- **Award type:** 5
- **Project period:** 1982-07-01 → 1990-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155641

## Citation

> US National Institutes of Health, RePORTER application 3155641, STRUCTURE-FUNCTION RELATIONS IN MYOSIN (5R01AR028401-07). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3155641. Licensed CC0.

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