# BONE CELLS AND VITAMIN D3

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1988 · $91,902

## Abstract

This proposal is a resubmission of a previous grant (AM 28420-04)
which was reviewed in June, 1986.

This proposal deals with the metabolism and effects of vitamin D
metabolites on isolated bone cells.  The specific aims have been
refocused 1) to elucidate the role of age on the extra renal
metabolism of 25-hydroxy-vitamin D (25(OH)D3) by bone cells, 2) to
study the effect of exogenous stimulators of proliferation and
differentiation on the metabolism of 25(OH)D3, 3) to refine a cell
isolation technique to the point of identifying which bone cell
phenotype is responsible for the metabolism of 25(OH)D3 and 4) to
purify and study the effect of hormones on bone cell
phosphotyrosine phosphatase (PTPase).  The long term objectives of
this work are to determine whether extra renal metabolism of
vitamin D metabolites by bone cells is physiologically important.
Moreover, this work will also explore the role of calcitropic
hormones in regulating cell proliferation by their action on the
newly discovered enzyme, PTPase.

Since the previous submission of this proposal new information
regarding the regulation of a chick bone cell 25(OH)D3-1-
hydroxylase has been obtained.  This enzyme appears to be regulated
in a complex way by vitamin D metabolites and has been shown to
have a short half-life (approximately 5 hours).

## Key facts

- **NIH application ID:** 3155647
- **Project number:** 2R01AR028420-04A3
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** J. Edward PUZAS
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $91,902
- **Award type:** 2
- **Project period:** 1982-02-01 → 1991-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155647

## Citation

> US National Institutes of Health, RePORTER application 3155647, BONE CELLS AND VITAMIN D3 (2R01AR028420-04A3). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3155647. Licensed CC0.

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