# THE SEQUENCING OF CHONDROITIN S04 AND KERATAN S04

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 1987 · $90,304

## Abstract

Chondromucoproteins (CMP) synthesized by chick embryo chondrocytes contain
three types of carbohydrates which are O-linked to a large core protein.
These include chondrointin sulfate chains (CS), keratan sulfate chains
(KS), and O-linked oligosaccharides.  Methods will be developed to
determine the compositions and monomer sequences in each of these types of
structures and the relative amount of each structure in the several species
of CMP that are synthesized by the chondrocytes.  For sequencing the CS
chains, a series of oligosaccharides, each with a linkage region sequence
at its reducing terminal and a Ga1NAc, Ga1NAc-4SO4, or Ga1NAc-6SO4 at its
nonreducing terminal, will be isolated.  The oligosaccharides will be
analyzed to determine their nonreducing terminal residue(s) as a function
of increasing distance from the linkage region.  Similarly, KS will be
sequenced by preparing a series of oligosaccharides, each with the YS
linkage region at its reducing terminal and a G1cNAc or G1cNAc-6SO4 residue
at its nonreducing terminal.  The oligosaccharides will be analyzed to
determine their nonreducing terminal residue(s) as a function of increasing
distance from the linkage region.  These methods will be used to analyze
the structural differences in the different CMP's produced by the
chondrocytes and the structural changes in these O-linked carbohydrates
that occur when culture conditions are varied.  It is a long term goal of
these studies to correlate these structures with the activities of the
CMP's in cartilage calcification.

## Key facts

- **NIH application ID:** 3155691
- **Project number:** 5R01AR028588-07
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **SAM.gov UEI:** Y8CWNJRCNN91
- **Principal Investigator:** H. EDWARD CONRAD
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $90,304
- **Award type:** 5
- **Project period:** 1981-07-01 → 1988-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155691

## Citation

> US National Institutes of Health, RePORTER application 3155691, THE SEQUENCING OF CHONDROITIN S04 AND KERATAN S04 (5R01AR028588-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155691. Licensed CC0.

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