# HORMONE REGULATION OF BONE COLLAGEN SYNTHESIS

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 1991 · $194,179

## Abstract

We propose to analyze the DNA sequences and protein binding factors which
are responsible for elevated transcription of the alpha-1(I) collagen gene
in bone cells as compared to fibroblast, and also these sequences and
factors responsible for the inhibition of alpha-1(I) synthesis by vitamin
D in bone cells but not fibroblasts.  We will follow-up on our preliminary
evidence that there is a bone-specific enhancer between -3521 and -2291,
and a vitamin D responsive element between -2291 and -1665 upstream from
the RNA transcription start site.  We will analyze DNA sequences using
deletions and oligonucleotide directed mutagenesis followed by analysis of
transcriptional activity and hormone regulation of the mutants in stably
transfected osteosarcoma and fibroblast cell lines, and also in transgenic
mice.  Subfragments of the promoter will be tested for their effect on the
transcription of a weak heterologous promoter.  In vitro protein/DNA
interactions will be analyzed by gel mobility shifts and footprinting using
nuclear extracts from hormone treated and control bone cells and
fibroblasts.  In vivo chromatin structure will be analyzed using DNase
sensitivity studies.  Appreciating the different regulatory influences on
collagen production in bone and fibroblast is essential for understanding
genetic acquired disorders of bone.

## Key facts

- **NIH application ID:** 3155730
- **Project number:** 2R01AR029983-08A3
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **SAM.gov UEI:** H6D6JMXJXDE6
- **Principal Investigator:** Alexander C Lichtler
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1991
- **Award amount:** $194,179
- **Award type:** 2
- **Project period:** 1981-08-01 → 1994-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155730

## Citation

> US National Institutes of Health, RePORTER application 3155730, HORMONE REGULATION OF BONE COLLAGEN SYNTHESIS (2R01AR029983-08A3). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155730. Licensed CC0.

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