# STUDIES OF EPIDERMAL MACROMOLECULAR METABOLISM

> **NIH NIH R01** · NEW YORK UNIVERSITY · 1987 · $333,621

## Abstract

During the next five years, we will continue to study epidermis and
epidermal appendages in health and disease by focusing on keratin gene
expression in a number of model systems.  During this period, we shall use
guinea pig epidermis, mouse hair root cells, cell lines such as ME 180
derived from pathologic human tissue and human epidermal biopsies to
identify and characterize the keratin genes and to prepare radiolabelled
probes of the study of gene expression.  Probes will be used to analyze a
number of physiologic states, such as mitosis and againg, a number of
pathologic conditions, such as tumors, psoriasis and the several
ichthyoses, and a group of pharmacologic agents, such as Vitamin A and
corticosteroids.  Ultimately, we will use the clones and probes developed
to determine the nucleotide sequences of keratin cDNA, and the amino acid
sequences of keratin in normal and disease states.  In parallel, we will
investigate the glycosylation and phosphorylation of keratin and other
intermediate filament proteins in the models we have noted.  We will use
these data to identify and localize subunits of keratins in cells and
tissue from normal and pathologic epidermis.  The molecular biologic and
biochemical evidence developed during these investigations should lead to a
definition of the function of keratin and other intermediate filament
proteins and to data concerning the relationships among keratin and other
cellular constitutents.

## Key facts

- **NIH application ID:** 3155867
- **Project number:** 5R01AR030682-07
- **Recipient organization:** NEW YORK UNIVERSITY
- **SAM.gov UEI:** NX9PXMKW5KW8
- **Principal Investigator:** IRWIN M FREEDBERG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1987
- **Award amount:** $333,621
- **Award type:** 5
- **Project period:** 1981-07-01 → 1989-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155867

## Citation

> US National Institutes of Health, RePORTER application 3155867, STUDIES OF EPIDERMAL MACROMOLECULAR METABOLISM (5R01AR030682-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155867. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*