# STRUCTURE AND METABOLISM OF VASCULARIZED ALLOGRAFTS

> **NIH NIH R01** · CASE WESTERN RESERVE UNIVERSITY · 1988 · $278,819

## Abstract

This proposal extends research into the biology of vascularized
and nonvascularized bone grafts in a canine model in continuation
of a long-term study, and in light of recent findings.  Autografts
and allografts, vascularized and nonvascularized, will be
exchanged across carefully defined histocompatibility barriers.
Cyclosporin-A will be further evaluated as an immunosuppressant
in this context.  Vascularized allografts, carefully matched at the
major histocompatibility site, will be studied for their
mechanical, morphological and biochemical properties at 3 and 6
months post-transplantation.  The role of the Class I and Class II
histocompatibility antigens will be evaluated in terms of the
fates of both vascularized and nonvascularized grafts and also in
terms of the immune responses generated by each of these kinds
of grafts.  Finally, a study will be undertaken to determine
optimal parameters for the storage of bone destined for
vascularized grafting.

A unique feature of this project is the unusually thorough
evaluation which will describe intermediate and end-points of
acceptance of the grafts by their hosts.  Specifically, these
include (a) metabolic dynamics of bone calcium and collagen in
pre-labelled animals; (b) measurement of mechanical properties of
the grafts; (c) morphological evaluation by means of conventional
histology, quantitative histomorphometry, and radiography; (d)
immunological evaluation by means of conventional antibody and
cell-mediated immunity measurements, by matching and careful
control of genetic characteristics (MHC) of donor and recipient,
and by use of selective methods of immunosuppression.
Correlation of the first three parameters with each other as
functions of the immunological measurements should provide
useful perspectives in relating each of these parameters to the
host response to the graft.

In view of the very slow total acceptance of bone grafts, studies
such as these should be extended over a longer period of time to
yield the ultimate data on the fate of the grafts.  This present
proposal comprehensively evaluating critical biological and
physical parameters should provide guidance for the future in
regard to the role vascularized bone transplantation may play in
clinical practice.

## Key facts

- **NIH application ID:** 3155900
- **Project number:** 5R01AR030833-07
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **SAM.gov UEI:** HJMKEF7EJW69
- **Principal Investigator:** VICTOR M GOLDBERG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $278,819
- **Award type:** 5
- **Project period:** 1982-09-30 → 1991-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155900

## Citation

> US National Institutes of Health, RePORTER application 3155900, STRUCTURE AND METABOLISM OF VASCULARIZED ALLOGRAFTS (5R01AR030833-07). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155900. Licensed CC0.

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