# MONOCLONAL ANTIBODIES TO HUMAN EPIDERMIS

> **NIH NIH R01** · UNIVERSITY OF ROCHESTER · 1988 · $201,300

## Abstract

Monoclonal antibodies to epidermal and basement membrane zone antigens have
been formed and new antibodies will be formed by fusing spleen cells from
immunized mice with the mouse myeloma cell line P3/NS1.  Mice immunized
with a human cervical carcinoma cell line (ME-180) have formed an antibody
to human anchoring fibrils.  The antigen in human skin and ME-180 will be
completely characterized with its molecular weight, isoelectric point,
peptide and cyanogen bromide map and amino acid composition.  The cell of
origin of the antigen in skin will be determined by biosynthetic studies
and dermal/epidermal recombinations on the nude mouse.  The formation of
the anchoring fibril and its antigen will be sequentially followed during
wound healing and during epidermal cell attachment to collagen-coated
tissue culture dishes.  Gene mapping of the anchoring fibril antigen will
be performed by somatic cell hybridization techniques using ME-180 cells
which will be fused with polyethylene glycol with mouse epidermal cells.
The anchoring fibril antigen has reacted with many samples of human skin
but is non-reactive with the non-blistered skin of patients with dystrophic
epidermolysis bullosa, a disease in which anchoring fibrils are absent as
shown by electron microscopy.  Attempts to use the anchoring fibril
antibody to follow therapy of epidermolysis bullosa will be made both by
biopsies of patients and by following the skin biopsies of the
epidermolysis bullosa skin transplanted to the nude mouse.  Success with
these techniques encourages us to use these techniques to form antibodies
which will allow dissection of the heterogeneous population of epidermal
basal cells and also which distinguishes basal from non-basal cells.

## Key facts

- **NIH application ID:** 3155935
- **Project number:** 5R01AR030965-08
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **SAM.gov UEI:** F27KDXZMF9Y8
- **Principal Investigator:** LOWELL Alan GOLDSMITH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $201,300
- **Award type:** 5
- **Project period:** 1981-08-01 → 1990-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3155935

## Citation

> US National Institutes of Health, RePORTER application 3155935, MONOCLONAL ANTIBODIES TO HUMAN EPIDERMIS (5R01AR030965-08). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3155935. Licensed CC0.

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