# IMMUNOBIOCHEMICAL STUDY OF MUSCLE MYOSIN ISOFORMS

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA AT DAVIS · 1988 · $121,122

## Abstract

The objective is to determine what factors regulate the expression of
myosin heavy chain isoforms during chicken skeletal muscle differentiation
and maturation.  Our immediate aim is to identify and characterize myosin
heavy chain isoforms found in fast and slow skeletal muscles at various
stages of normal development.  Our studies will include a combination of
biochemical and immunological techniques and employ the numerous monoclonal
antibodies that we have prepared during the first three years of this
project.  Our initial studies have shown that there are more myosin heavy
chain isoforms than previously believed and different developmental
programs exist among fast muscle fibers.  Thus we will continue to screen
for monoclonal antibodies which can distinguish the closely related fast
and the closely related slow developmental isoforms.  We will compare the
program of myosin heavy chain expression during the development of other
fast and slow muscles and fast and slow muscle fibers to what we have
already observed in the pectoralis major, the posterior latissimus dorsi,
the anterior latissimus dorsi, and the lateral gastrocnemius.  In order to
understand the regulation of myosin isoform expression we will examine the
myosin transitions that occur during muscle regeneration following cold
injury or Marcaine injection in normally innervated and denervated
muscles.  We will also study the myosin changes that may accompany muscle
degeneration and atrophy following denervation and/or limb immobilization.
We will determine what myosins are expressed following hypertrophy in
chicken wing muscles.  We will also determine the effect of the
administration of various hormones on myosin transitions during normal
development and during the altered growth conditions described above.
Finally, we will continue to study myosin expression of muscle cells in
culture in order to determine appropriate conditions to induce myosin heavy
chain transitions and muscle fiber maturation in vitro.

## Key facts

- **NIH application ID:** 3156073
- **Project number:** 5R01AR031731-06
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **SAM.gov UEI:** TX2DAGQPENZ5
- **Principal Investigator:** EVERETT BANDMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $121,122
- **Award type:** 5
- **Project period:** 1983-01-01 → 1988-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156073

## Citation

> US National Institutes of Health, RePORTER application 3156073, IMMUNOBIOCHEMICAL STUDY OF MUSCLE MYOSIN ISOFORMS (5R01AR031731-06). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156073. Licensed CC0.

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