# ION CHANNELS AND CALCIUM RELEASE IN MUSCLE

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 1992 · $127,616

## Abstract

The long-term goal of this project is to understand the cellular mechanisms
that influence the release of calcium from the sarcoplasmic reticulum
(s.r.) of skeletal muscle.  As the level of force production in muscle
depends on the concentration of cytoplasmic calcium reached process is
essential to understanding how muscle cells are activated both normally and
in diseased states.

The work will investigate the role of protein phosphorylation in regulating
calcium release channel activity.  The specific aims are 1) to investigate
the influence of phosphorylation on the activity of single s.r. calcium
release channels and 2) to determine whether phosphorylation plays a
physiologically important role in regulating calcium release from single
muscle cells.  Additional experiments are designed to describe the
conductance selectivity and gating properties of the s.r. calcium channel
under physiological conditions.

The project will use two basic experimental approaches.  Single channel
patch clamp recording will be used to study the behavior of the calcium
release channel from frog skeletal muscle fibers.  The s.r. membrane will
be exposed by removing the surface membrane of isolated single fibers and
then causing the fiber to contract to very short lengths.  Under these
conditions s.r. membrane is extruded from the surface of the fiber and can
be approached with patch microelectrodes.  The response of a more intact
preparation will also be studied using tension generation or calcium
indicator dyes to monitor calcium release from single cells ("skinned
fibers").

## Key facts

- **NIH application ID:** 3156169
- **Project number:** 2R01AR032062-12A1
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **SAM.gov UEI:** Y8CWNJRCNN91
- **Principal Investigator:** PHILIP M BEST
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1992
- **Award amount:** $127,616
- **Award type:** 2
- **Project period:** 1979-12-01 → 1996-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156169

## Citation

> US National Institutes of Health, RePORTER application 3156169, ION CHANNELS AND CALCIUM RELEASE IN MUSCLE (2R01AR032062-12A1). Retrieved via MORVS 2026-10-25 from https://api.morvs.ai/grant/nih/3156169. Licensed CC0.

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