# MYOFIBRIL ASSEMBLY IN STRIATED MUSCLE

> **NIH NIH R01** · WEILL MEDICAL COLL OF CORNELL UNIV · 1988 · $250,664

## Abstract

Myosin, the predominant protein within the myofibrils of skeletal and cardiac
muscle occurs in several isomorphic (isoenzymic) forms.  Evidence is
accumulating that early in development two or more of these isomorphs can
coexist within the same cells, but with progressive development the individual
myosin types become segregated into their characteristic adult locations.
Detection of myosin isoenzymes is often done immunologically with appropriately
cross-absorbed xenogenic antisera.  Problems have often arisen with the
specificity of these antisera and uncertainties remain about the number of
different structural genes for myosin heavy and light chains which are truly
expressed in different muscle cells.  Also, it is unclear if different myosin
isomorphs coassemble within common myofibrils, sarcomeres and thick filaments.
Using the newly developed technique of Kohler and Milstein (1975), Nature
256:495, we have generated 18 hybridoma lines which secrete monoclonal
antibodies to myosin isolated from adult chick breast muscle.  Three of these
antibody preparations for breast myosin where as the other 15 crossreact to
varying degrees with other myosin isoenzymes.  In this application, we propose
to generate additional monoclonal antibodies specific to the adult and embryonic
myosins from cardiac, ALD and pectoralis major muscles of the chicken.
Specificities and binding affinities will be compared by radioimmune assay and
immunofluorescence.  Location of antigenic determinants within myosin molecules
will be established by antibody binding to denatured subunits, proteolytic
fragments of heavy chains and electron microscopic immunocytochemistry.
Detection of isomorphic variants with embryonic heart and skeletal muscle will
then be performed by double label immunofluorescence with cell cultures and
frozen sections of embryonic material.

## Key facts

- **NIH application ID:** 3156203
- **Project number:** 5R01AR032147-07
- **Recipient organization:** WEILL MEDICAL COLL OF CORNELL UNIV
- **SAM.gov UEI:** YNT8TCJH8FQ8
- **Principal Investigator:** DONALD A FISCHMAN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 1988
- **Award amount:** $250,664
- **Award type:** 5
- **Project period:** 1982-07-01 → 1989-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/3156203

## Citation

> US National Institutes of Health, RePORTER application 3156203, MYOFIBRIL ASSEMBLY IN STRIATED MUSCLE (5R01AR032147-07). Retrieved via MORVS 2026-10-24 from https://api.morvs.ai/grant/nih/3156203. Licensed CC0.

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